Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de
Background and objectives: Campylobacter infections still are an issue of major importance worldwide. Different in vivoapproaches to reduce Campylobacter infectionsdid not prove sufficient. For this reason, we aim to examine the effect of different non-biosecurity based measures on Campylobacter prevalence in animal trials.Material and Methods: A Seeder-bird model was established to assess the impact of non-biosecurity based measures on Campylobacter prevalence. Broilers of breed ROSS 308 are fed with standard diet and retained on ground floor with litter and a stocking density of 39 kg/m².In each trial we orally challenge 18 (seeders) out of 90 broilers with 104colony forming units of a C. jejuni reference strain. Successful colonization of the seeder is checked once by taking cloacal swabs 48 hours after inoculation. During the trial 36 randomly selected untreated broilers (sentinels) are tested for Campylobacter colonization and load by taking cloacal swabs at predetermined intervals. At the end of each trial after necropsy we semiquantitatively analyse the intestinal content (ceacum and colon) for C. jejuni.To examine the effect of carvacrol the treated group was fed daily with 120mg/kg feed of carvacrol.Results:Our first results show that the C. jejuniload in the carvacrol treated group (mean value 4,19 log 10 MPN/g)is significantly lower compared to the controlgroup (mean value 5,16 log 10 MPN/g). The final results of the ongoing project will be presented.