Publikationsdatenbank

Are ESBL- producing E. coli spread in the environment by manure application, soil cultivation and wind erosion? (2019)

Art

Vortrag

Autoren

Siller, Paul (WE 10)

Dähre, Katrin (WE 10)

Rösler, Uwe (WE 10)

Kongress

National Symposium on Zoonoses Research 2019

Berlin, 16. – 18.10.2019

Quelle

Zoonoses 2019 - International Symposium on Zoonoses Research : Book of Abstracts — International Symposium on Zoonoses Research (Hrsg.)

Berlin, 2019 — S. 53

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/79/attachments/23/154/Book_of_Abstracts_Zoonoses2019.pdf

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives:
Broiler and pig manure frequentlycontains ESBL-producing E. coli and is used to fertilize arable land. To estimate the risk of an airborne spread of these resistant bacteria from organic fertilizers to the environment, we performedfield, wind channel andshort-term manure storage trials.

Materials and Methods:
In field trials, we took air and soil samples while and after ESBL-containing fertilizer application and agricultural cultivation. In wind channel trials, an ESBL-positive soil-manure mix was exposed to different wind speeds to simulate wind erosion. In manure storage trialsin summer and winter, the number of ESBL-E. coli was monitored in a chicken manure pile. All samples were qualitatively and quantitatively analysed. Selected isolates were analysedby NGS.

Results:
In the field and wind channel experiments, ESBL-E. coli were not detected in the air samples. In onefield trial with pig slurry, ESBL-E. coliwere detected in soil samples after 33 days. In the manure storage trials we saw an immediate and rapid decline in ESBL-E. colinumbers.

Conclusion:
The amount of ESBL-E. coliin organic fertilizers drops immediately after leaving the stables. When incorporated to soil, they can survive for at least one month, butESBL-E. coliseem to have a low survival rate when aerosolized. Storing manure before land application is effective to reduce bacterial counts. Considering all results, an airborne environmental spread seems unlikely.