Publikationsdatenbank

How likely is an environmental spread of ESBL–producing E. coli out of manure? (2019)

Art

Vortrag

Autoren

Siller, Paul (WE 10)

Daehre, Katrin (WE 10)

Roesler, Uwe (WE 10)

Kongress

XIX International Congress of ISAH 2019

Wrocław, Poland, 08. – 12.09.2019

Quelle

Proceedings of the XIXth International Congress of the International Society for Animal Hygiene : animal hygiene as a fundament of one health and welfare improving biosecurity, environment and food quality — International Society for Animal Hygiene (Hrsg.)

Wrocław, Poland: University of Environmental and Life Sciences, 2019 — S. 154

Sprache

Englisch

Verweise

URL (Volltext): https://www.isah-soc.org/?Events/Congresses/2019-Poland

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Resistant bacteria in the environment are a “One Heath” problem. Broiler and pig manure, which is often tested positive for ESBL-producing Enterobacteriaceae is used to fertilize arable land. We aim to assess the risk of an airborne spread of resistant bacteria to the environment by wind channel trials. We also evaluated an on-farm mitigation strategy, therefore we performed short-term manure storage trials in summer and winter.
In the wind channel trials, ESBL-positive manure was mixed with soil and exposed to different wind speeds to simulate wind-driven erosion. Air samples were taken via impingement and automated impingement. For the manure storage trials superficial and deep manure samples were taken over five consecutive days from a stockpiled manure heap, to investigate decline rates of ESBL-producing E. coli in winter and in summer. All samples were qualitatively and
quantitatively analyzed by cultivation. Species-identification was performed with MALDI-TOF and resistance genes were analyzed via real-time PCR and subsequent sequencing.
In the wind channel experiments a detection of ESBL-producing E. coli in the air samples was impossible despite high concentrations in the used manure (up to 4,2*105 CFU/g of manure). Non-resistant E. coli were detected in 11/60 air samples. In the manure storage trials the number of ESBL-producing E. coli decreased continuously. A qualitative detection was impossible for 3/6 deep manure samples taken after 72h and 96h respectively in the winter trial and
6/6 samples in the summer.
Our results indicate, that an airborne environmental spread of ESBL-producing E. coli cannot be excluded, but has to be considered unlikely, at least for large quantities of these resistant microorganisms. We have also shown, that an extended storage period before usage as organic fertilizer is effective for reducing the number of ESBL-producing E. coli in manure.