Publikationsdatenbank

Minimum Inhibitory Concentrations for glyphosate and glyphosate-containing formulation in livestock-associated Enterobacteriaceae (2019)

Art

Vortrag

Autoren

Bote, Katrin (WE 10)

Pöppe, Judith (WE 10)

Makarova, Olga (WE 10)

Merle, Roswitha (WE 10)

Roesler, Uwe (WE 10)

Kongress

XIX International Congress of ISAH 2019

08. – 12.09.2019

Quelle

Proceedings of the XIXth International Congress of the International Society for Animal Hygiene : animal hygiene as a fundament of one health and welfare improving biosecurity, environment and food quality — International Society for Animal Hygiene (Hrsg.)

Wrocław, Poland: University of Environmental and Life Sciences, 2019 — S. 86–87

Sprache

Englisch

Verweise

URL (Volltext): https://www.isah-soc.org/?Events/Congresses/2019-Poland

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Glyphosate is the most popular herbicide in the world. The target structure of glyphosate, the enolpyruvylshikimate-3-phosphate synthase (EPSPS), is also present in bacteria. Concerns have been raised about the potential effects of glyphosate residues in animal feed on bacteria in livestock. Here, we investigated the current levels of sensitivity to glyphosate (as an active ingredient and as a part of the formulation Roundup LB plus) in diverse isolates of
Escherichia coli and Salmonella enterica.
We determined minimum inhibitory concentrations (MIC) of glyphosate and Roundup LB plus in Müller-Hinton I medium using a broth microdilution method for 225 Salmonella spp. and 238 E. coli isolates. Statistical modelling was used to determine the effects of the different periods of isolation, host species, serovars, pathogenicity and ESBL-status of isolates on susceptibility to glyphosate/RU.
The distribution of MIC values for Salmonella spp. was 10-80 mg/ml for glyphosate and 20-80 mg/ml for RU. For E. coli, the distribution of MIC values was 5-10 mg/ml for glyphosate and 20-40 mg/ml for formulation. Among the isolates with a MIC higher than the 95%-percentile, the pathogenic E.coli were the dominant subgroup, in Salmonella spp. no population over the 95%-percentile was identified. Antibiotic resistance profile did not have a large effect on glyphosate sensitivity in E. coli, whereas host animal species and periods of isolation had small but statistically significant effects on glyphosate MICs in both species.
These results demonstrate that although Salmonella spp are more resistant to glyphosate than E. coli, the MIC distribution within the species is small, suggesting the absence of a highly resistant subpopulation in farm animalassociated Enterobacteriaceae.