Publication Database

Resistance induction to Roundup, a common herbicide, in Enterobacteriaceae in vitro (2019)

Art

Vortrag

Autoren

Pöppe, Judith (WE 10)

Bote, Katrin (WE 10)

Roesler, Uwe (WE 10)

Makarova, Olga (WE 10)

Kongress

XIX International Congress of ISAH 2019

08. – 12.09.2019

Quelle

Proceedings of the XIXth International Congress of the International Society for Animal Hygiene : animal hygiene as a fundament of one health and welfare improving biosecurity, environment and food quality — International Society for Animal Hygiene (Hrsg.)

Wrocław, Poland: University of Environmental and Life Sciences, 2019 — S. 86

Sprache

Englisch

Verweise

URL (Volltext): https://www.isah-soc.org/?Events/Congresses/2019-Poland

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Glyphosate is the most widely used herbicide in the world, and its formulation Roundup LB plus (RU) is the most common glyphosate-containing herbicide in Germany. Antimicrobial effects of glyphosate have recently been recognised. The objective of this study was to investigate the ability of RU to induce resistance in Enterobacteriaceae of animal origin as well as cross-resistance to antibiotics and fitness costs in the resulting resistant mutants.Ten isolates each of Escherichia coli and Salmonella enterica serovars from pigs and cattle were passaged daily at increasing concentrations of RU. Whole genome sequencing has been performed for the stable resistant isolates and respective ancestors. Fitness costs were assessed by growth curves and cross-resistance to antibiotics determined by Vitek.The overall dynamics of adaptation to RU was slow and relatively low-level, with early extinctions in E. coli. One E. coli and four Salmonella isolates showed a 2-4-fold increase in minimum inhibitory concentration (MIC) to RU. Mutations associated with glyphosate resistance were not found in E. coli but in all Salmonella isolates. There were no changes in fitness costs or antibiotic resistance profiles.Salmonella are more likely to develop resistance to RU compared to E. coli. Resistant mutants show no fitness costs. RU resistance does not occur easily and is relatively low-level. It is therefore possible that RU use may result in preferential selection of pathogenic RU-tolerant Salmonella bacteria that can persist in the environment.