Publikationsdatenbank

More than just a weed killer:
exposure to Roundup selects for resistance with low fitness costs in pathogenic enteric bacteria Escherichia coli and Salmonella enterica from farm animals (2019)

Art

Vortrag

Autoren

Bote, K. (WE 10)

Poeppe, J. (WE 10)

Johnston, Paul

Rösler, U. (WE 10)

Makarova, O. (WE 10)

Kongress

Zoonoses 2019 – International Symposium on Zoonoses

Berlin, 16. – 18.10.2019

Quelle

Zoonoses 2019 - International Symposium on Zoonoses Research : Book of Abstracts — International Symposium on Zoonoses Research (Hrsg.)

Berlin, 2019 — S. 244

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/79/attachments/23/154/Book_of_Abstracts_Zoonoses2019.pdf

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives:
The herbicide glyphosate, the active ingredient in the formulation Roundup (RU), is currently the most-used herbicide in the world. Additionally, it is patented as an antimicrobial and its effects on microbes are currently investigated. Both Escherichia coli (E. coli) and Salmonella entericaare important zoonotic Enterobacteriaceawhich can be exposed to glyphosate residues via feed. Therefore, we investigated whether RU can induce bacterial resistance and if it impacts their fitness.

Materials and methods:
Ten isolates each of E. coli and Salmonellaentericaserovars from pigs and cattle were passaged daily at increasing concentrations of RU. Stable resistant isolates and respective ancestorswerewhole genome sequenced and growth dynamics analysed.

Results:
The overall dynamics of adaptation to RU was slow and relatively low-level, with early extinctions in E. coli. One E. coli and four Salmonella isolates showed a 2-4-fold increase in minimum inhibitory concentrationto RU. Mutations associated with glyphosate resistancewere found in all Salmonella isolates but not in E. coli. Mostly no fitness costswere found.

Conclusion:
Salmonella are more likely to develop resistance to RU compared to E. coli. Although RU resistance does not occur easily and is relatively low, resistant mutants show no fitness costs. This suggests that RU may result in preferential selection of pathogenic Salmonella bacteria that can persist in the environment.