Publikationsdatenbank

Advanced imaging techniques:
microendoscopy and fluorescence lifetime imaging (2019)

Art

Vortrag

Autor

Niesner, Raluca A. (WE 2)

Kongress

14th European Molecular Imaging Meeting

Glasgow, 19. – 21.03.2019

Quelle

Sprache

Englisch

Verweise

URL (Volltext): https://www.eventclass.org/contxt_emim2019/online-program/session?s=ES+04#e898

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

In the last three decades, two-photon excitation laser-scanning fluorescence microscopy proved to be the most powerful tool to monitor typical cell motility patterns and cell communication in living mamals. Still there are several limitations concerning the accessible organ areas, the number of simultaneously
distinguishable tissue compartments as well as cellular and tissue function. In order to visualize the currently inaccesible deep marrow cavity of longe bones - as the site of cell birth and of immune memory - we employed a gradient refractive lens system and, thus, developed a microendoscopic approach for longitudinal in vivo femoral imaging [1]. Similar approaches have been previously used for brain cortex imaging, especially in the lab of Mark Schnitzer. Various two-photon microendoscopic techniques will be discussed and compared with state-of-the-art two-photon microscopy as far as the optical performance is concerned. In order to grasp the complexity of biological processes, to which several cellular and noncellular tissue compartments are contributing, we developed multiplexed intravital imaging by using a synergistic strategy of wave mixing two-photon excitation and non-algebraic color-unmixing algorithms
[2]. Thus, we could simultaneously visualize up to 8 components of the lymph node, during germinal center reactions, over time. Last but not least, the power of fluorescence lifetime imaging in intravital microscopy is revealed on the example of NADH and NADPH-dependent metabolism and cellular Calcium monitoring during phagocytosis followed by NETosis [3] and during chronic neuroinflammation [4,5].