Publikationsdatenbank

Co-registered spectral optical coherence tomography and two-photon microscopy for multimodal deep-tissue imaging in adult mice (2019)

Art

Vortrag

Autoren

Rakhymzhan, Asylkhan

Reuter, Lucie

Raspe, Raphael

Bremer, Daniel

Günther, Robert

Leben, Ruth

Heidelin, Judith

Andresen, Volker

Spiecker, Heinrich

Hauser, Anja

Radbruch, Helena

Hüttmann, Gereon

Schulz-Hildebrandt, Hinnerk

Niesner, Raluca (WE 2)

Kongress

29. Jahrestagung der Deutschen Gesellschaft für Zytometrie

Berlin, 25. – 27.09.2019

Quelle

Visions in Cytometry : Microscopy, Multiplexing Analysis, Flow-/Mass-Cytometry : 29th Annual Conference of the German Society for Cytometry : Abstract booklet — Deutsche Gesellschaft für Zytometrie (Hrsg.)

Berlin: DGfZ, 2019 — S. 22

Sprache

Englisch

Verweise

URL (Volltext): http://www.dgfz.org/blog/wp-content/uploads/2020/02/2019_DGfZ_Abstract_Booklet.pdf

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Intravital two-photon microscopy (2PM) brought in the last decades unique insight into the mechanisms of immune system dynamics since it enables monitoring of cellular motility and communication of complex systems, in genuine environment. Optical coherence tomography (OCT), with broad application both in research and clinics, is a non-invasive diagnostic method which allows monitoring morphologic changes over large tissue regions. Hence, OCT represents a bridge from bench to bed-side. Here we developed a combined multimodal technology to achieve for the first time co-registered OCT, 2PM and second harmonic generation (SHG) imaging over large volumes (1x1x0.3 mm³) of the paw, spleen, lymph node and femur in mice. We found that processes of resident macrophages in the tendons of the hind paw continuously probe the tissue following the extracellular matrix structures revealed by OCT and SHG. The new design of the combined OCT and 2PM approach opens new roads for multimodal in vivo imaging correlated with dynamic information of various, hardly accessible organs in adult mice.