Publikationsdatenbank

Quercetin enhances barrier function in porcine jejunum but not in follicle-associated epithelium of Peyer’s patches (2019)

Art

Poster

Autoren

Cornelius, V. (WE 2)

Amasheh, S. (WE 2)

Kongress

98th meeting of the German Physiological Society

Ulm, 30.09. – 02.10.2019

Quelle

Acta physiologica Scandinavica

Bandzählung: 227

Heftzählung: S719

Seiten: 134

ISSN: 0001-6772

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/toc/17481716/2019/227/S719

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Question:
Previous studies on human cell lines like Caco-2 [1] or HT29-B6 [2] have shown a barrier-strengthening effect of the plant flavonoid quercetin. Due to the different barrier properties of jejunal villous epithelium (VE) and the follicle-associated epithelium (FAE) of Peyer ́s patches (PP) [3], we performed Ussing chamber experiments to analyze the effects of quercetin on both jejunal epithelium types.

Methods:
Epithelial tissue samples of distal jejunum with and without PP were mounted into Ussing chambers and quercetin was added to the mucosal side (0, 2, 20, 200 and 400 μM). During 4 h of incubation, transepithelial electrical resistance (TEER) was recorded. In a second approach, unidirectional tracer flux measurements were performed with [3H]-mannitol. Subsequently, claudins were analyzed via rt-qPCR and immunoblotting. Statistical analysis was performed using repeated measures ANOVA and Dunnett-test for multiple comparison for TEER and flux results and unpaired Student ́s t-test for densitometry and rt-qPCR. Values below p = 0.05 were considered to be statistically significant.

Results:
In VE, 400 μM quercetin showed an effect on TEER (p< 0.01, n=24), whereas mannitol flux did not changesignificantly (n=13). Analysis of claudins revealed an increase of claudin-4 in Western blots (p<0.01, n=6) and a 1.7-fold expression in rt-qPCR (p<0.05, n=4). The protein level of claudin-2 was reduced to 0.53 (p<0.01, n= 5) whereas the mRNA-expression was not changed (n=4). Quercetin had no significant effect on PP.

Conclusion:
In our study quercetin only had an effect on the intestinal barrier of porcine VE. The higher TEER values can be explained by both, an upregulation of tightening claudin-4, and a decrease of pore-forming claudin-2. The lower susceptibility of PP FAE to effects of luminal bioactive compounds might be attributed to selective immunological functions.