Publikationsdatenbank

A menthol-containing feed additive induces transcriptomic changes in sheep ruminal epithelium (2019)

Art

Poster

Autoren

Schulte, Jasper N. (WE 2)

Braun, Hannah-Sophie

Geiger, Sebastian (WE 2)

Patra, Amlan K. (WE 2)

Aschenbach, Jörg R. (WE 2)

Kongress

International Symposium on Ruminant Physiology (ISRP 2019)

Leipzig, 03. – 06.09.2019

Quelle

Advances in Animal Biosciences

Bandzählung: 10

Heftzählung: 3

Seiten: 527

ISSN: 2040-4719

Sprache

Englisch

Verweise

URL (Volltext): https://www.cambridge.org/core/services/aop-cambridge-core/content/view/7D464B8882B4D5304683797665CD9E5E/S2040470019000037a.pdf/proceedings_of_the_xiiith_international_symposium_on_ruminant_physiology_isrp_2019.pdf

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

RNA-sequencing is rapidly evolving and becoming the new gold standard to more accurately and comprehensively measure gene expression during transcriptome analysis. So far, only a few studies have been conducted regarding the global expression analysis in the ruminal epithelium of sheep. Therefore, MACE-seq (Massive Analysis of cDNA Ends), an improved variant of 3 ́ single end mRNA sequencing, was used to identify differentially expressed genes (DEG) and clusters within the ruminal epithelium of sheep fed either a control diet or supplemented with a menthol-based feed additive. In previous studies, both topical application of menthol to the ruminal epithelium or a menthol-supplemented diet were shown to modulate epithelial nutrient transport in the rumen. In the present study, fifteen female and nine male growing Suffolk sheep were evenly distributed to three dietary treatments including a randomized block design based on initial body weight and sex. The diets consisted of either no plant bioactive lipid compounds (PBLC; Control), a low dose of PBLC (80 mg/d, menthol-based OAX17, PerformaNat GmbH, Germany) or a high dose of PBLC (160 mg/d). After RNA isolation, DNase treatment, cDNA synthesis, PCR, and subsequent MACE-seq, raw reads were mapped and then annotated to the ENSMBL genome version 3.1 of Ovis aries (Version 3.1.9.23). Raw reads were normalized and p-values for the likelihood of differential gene expression were calculated. GO (Gene Ontology) enrichment analysis was performed to compare the control group with the combined and averaged PBLC-treated groups. Results showed 409 DEGs in Control versus both PBLC groups (209 upregulated, 200 downregulated) and GO enrichment analysis unveiled 132 significantly enriched GO terms among “Biological Process”, 29 among “Molecular Function” and 18 among “Cellular Compartment” (p < 0.05). Within the group of DEGs, genes related to intra- and extracellular transport, cell -cell adhesion, Ca2+ regulation, or cell cycle, among others, were strongly up- or downregulated (-1 < log2FC > 1), e.g. SLC6A17, SLC12A9, SLC25A46, SLC38A7, SCN7A, LRRC8A, CLDN3, S100A16, CDK8, and CUL4B. These results indicate that menthol seems to influence a vast number of genes and signaling pathways that might be beneficial to ion transport, energy homeostasis, and the desquamation of the ruminal epithelium. To our best knowledge, this is the first study evaluating changes in the ruminal transcriptome upon PBLC feeding. The identified DEGs and GO terms may help to understand previously described effects of menthol on gene expression and may provide further insights into the underlying mode of action.