Publikationsdatenbank

Effects of GLP-1 on expression of functional genes in rumen epithelium and its association to concentrate intake (2019)

Art

Vortrag

Autoren

Lu, Zhongyan

Shen, Hong

Greco, Gabriele (WE 2)

Amasheh, Salah (WE 2)

Aschenbach, Jörg (WE 2)

Shen, Zanming

Kongress

International Symposium on Ruminant Physiology (ISRP 2019)

Leipzig, 03. – 06.09.2019

Quelle

Advances in Animal Biosciences

Bandzählung: 10

Heftzählung: 3

Seiten: 391

ISSN: 2040-4719

Sprache

Englisch

Verweise

URL (Volltext): https://www.cambridge.org/core/services/aop-cambridge-core/content/view/7D464B8882B4D5304683797665CD9E5E/S2040470019000037a.pdf/proceedings_of_the_xiiith_international_symposium_on_ruminant_physiology_isrp_2019.pdf

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background:
GLP-1 exerts multiple roles in gastrointestinal and metabolic physiology. Apart from its action as “ileal brake”, GLP-1 stimulates both pancreatic β-cell proliferation and inhibits β-cell apoptosis. Furthermore, GLP-1 improves endothelial dysfunction. Dietary nutrients induce GLP-1 release. Our previous studies showed diet-SCFA effects on gene expression in rumen epithelium. However, little is known about the role of GLP-1 in this process. The present study aimed at understanding the modulating effects of GLP-1 on the expression of functional genes in the ovine rumen epithelium and its relation to concentrate intake.

Materials and methods:
Two experiments were performed. In Exp1 ovine ruminal epithelia were incubated in Ussing chambers for 7 h with 25 nM or 250 nM of GLP-1 administered to the serosal side to test the mRNA abundances in three functional gene groups: 1) genes related to SCFA absorption, i.e., AE2, PAT1, MCT1, MCT4, NHE1, NHE3, and Na/K ATPase; 2) genes involved in the regulation of cell cycle progression, i.e., cyclin D1, cyclin E1, CDK2, CDK4, and CDK6, and genes involved in the regulation of cell apoptosis, e.g., Bax, Bcl2, caspase3, caspase8 and caspase9; 3) genes of cell connection, i.e., ZO-1, DSG-1 and Connexin-43. In Exp2, goats received a diet of dried hay:concentrate at 70%:30% (ML group) or 90:10 (LL group) for 28 d. Samples of rumen epithelium, ileum and colon were collected for mRNA analysis using real-time quantitative PCR.

Results:
Exp1: A concentration of 25 nM GLP-1 did not change the abundance of 73% of tested genes, but reduced the abundance of 23% of them. A concentration of 250 nM of GLP-1 enhanced the abundance for 71% of tested genes and inhibited 29% of them. These effect patterns appeared in each of three gene groups. Exp2: The blood concentration of GLP-1 was higher in ML than LL group. The expression of gene in rumen epithelium, including those 21 genes, being same as in Exp1, plus GLP-1 receptor increased in the ML group. The mRNA abundance of GLP-1 precursor proglucagon and prohormone convertase 1 was greater in the ML than in the LL group in ileum and colon.

Conclusion:
Concentrate feeding induces GLP-1 synthesis in the ileum and colon and GLP-1 receptor in the rumen epithelium. Concurrently, changes of functional gene expression occur in the rumen epithelium of goats. The stimulating effects of high-dose GLP-1 application to isolated ruminal epithelia ex vivo suggested that GLP-1 may contribute to these changes.