Publikationsdatenbank

A kaleidoscope of phenotypes:
mecC-positive Methicillin-resistant Staphylococcus aureus (MRSA) (2019)

Art

Poster

Autoren

Huber, Charlotte

Epping, Lennard

Stamm, Ivonne

Ziebuhr, Wilma

Marincola, Gabriella

Bischoff, Markus

Strommenger, Birgit

Cuny, Christiane

Witte, Wolfgang

Döllinger, Jörg

Schuadinn, Christoph

Semmler, Torsten

Lübke-Becker, Antina (WE 7)

Walther, Birgit

Kongress

Zoonoses 2019 - International Symposium on Zoonoses Research

16. – 18.10.2019

Quelle

Zoonoses 2019 - International Symposium on Zoonoses Research : Book of Abstracts — International Symposium on Zoonoses Research (Hrsg.)

Berlin, 2019 — S. 238

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/79/attachments/23/154/Book_of_Abstracts_Zoonoses2019.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

MRSA, harboring the resistance-mediating variantmecChave strong adaptive capabilities, which enable them to colonize humans as well as a wide range of wild-and domestic animals and cause opportunistic infections.
Here we report on 45mecC-MRSA,isolated from clinical samples of humanand animal origin in Europe. Phenotypic characterization including hemolysis assays, colony spreading, toxinand biofilm production abilities as well as proteomics were performed. Wholegenome sequences were investigatedusingMLST v-1.6, ResFinderv-2.1, VirulenceFinder v-1.6and Geneious 11.1.5.mecC-MRSA of animal origin were associated with six different host species, with most isolates being obtained from feline samples (73%). All isolates yielded Oxacillin minimum inhibitory concentrations between0.5 and <4 μg/ml. Phylogenetic analysis revealed closely related genomes harboringvarious mobile genetic elements carrying beyond others different enterotoxins, the toxic-shock-syndrome toxinas well as further virulence factors, clustering within four clonal complexes (CC): CC599, CC130, CC1943 and CC49. However, strains of each CC yielded unexpected phenotype variationsincluding hemolysis, colony spreading, toxin production and biofilm formation, reflecting putative differences in global regulatory systems.
Further research addressing the impact of phenotypic variation for the adaptive capability of the distinct mecC-MRSA lineages supporting theirubiquitous widespread occurrence is warranted.