Publication Database

Genomic and Functional Analysis of Emerging Virulent and Multidrug-Resistant Escherichia coli Lineage Sequence Type 648 (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Schaufler, Katharina (WE 7)

Semmler, Torsten

Wieler, Lothar H. (WE 7)

Trott, Darren J.

Pitout, Johann

Peirano, Gisele

Bonnedahl, Jonas

Dolejska, Monika

Literak, Ivan

Fuchs, Stephan

Ahmed, Niyaz

Grobbel, Mirjam

Torres, Carmen

McNally, Alan

Pickard, Derek

Ewers, Christa

Croucher, Nicholas J.

Corander, Jukka

Guenther, Sebastian

Quelle

Antimicrobial agents and chemotherapy

Bandzählung: 63

Heftzählung: 6

Seiten: Article e00243-19

ISSN: 1098-6596

Sprache

Englisch

Verweise

URL (Volltext): https://aac.asm.org/content/63/6/e00243-19

DOI: 10.1128/AAC.00243-19

Pubmed: 30885899

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The pathogenic extended-spectrum-beta-lactamase (ESBL)-producing Escherichia coli lineage ST648 is increasingly reported from multiple origins. Our study of a large and global ST648 collection from various hosts (87 whole-genome sequences) combining core and accessory genomics with functional analyses and in vivo experiments suggests that ST648 is a nascent and generalist lineage, lacking clear phylogeographic and host association signals. By including large numbers of ST131 (n = 107) and ST10 (n = 96) strains for comparative genomics and phenotypic analysis, we demonstrate that the combination of multidrug resistance and high-level virulence are the hallmarks of ST648, similar to international high-risk clonal lineage ST131. Specifically, our in silico, in vitro, and in vivo results demonstrate that ST648 is well equipped with biofilm-associated features, while ST131 shows sophisticated signatures indicative of adaption to urinary tract infection, potentially conveying individual ecological niche adaptation. In addition, we used a recently developed NFDS (negative frequency-dependent selection) population model suggesting that ST648 will increase significantly in frequency as a cause of bacteremia within the next few years. Also, ESBL plasmids impacting biofilm formation aided in shaping and maintaining ST648 strains to successfully emerge worldwide across different ecologies. Our study contributes to understanding what factors drive the evolution and spread of emerging international high-risk clonal lineages.