Publication Database

Plasmacytoid dendritic cell depletion modifies FoxP3+ T cell homeostasis and the clinical course of bacterial pneumonia in mice (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Lippitsch, Anne

Baal, Nelli

Chukovetskyi, Yuri

Cunningham, Sarah

Michel, Gabriela

Dietert, Kristina (WE 12)

Gurtner, Corinne

Gruber, Achim D. (WE 12)

Bein, Gregor

Hackstein, Holger

Quelle

Journal of leukocyte biology : JLB

Bandzählung: 106

Heftzählung: 4

Seiten: 977 – 985

ISSN: 1938-3673

Sprache

Englisch

Verweise

URL (Volltext): https://jlb.onlinelibrary.wiley.com/doi/full/10.1002/JLB.3AB0119-014RR

DOI: 10.1002/JLB.3AB0119-014RR

Pubmed: 31265764

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Plasmacytoid dendritic cells (pDC) are critical to antiviral defense because of their high production of type I IFNs; less is known regarding their functions in bacterial infection. Moreover, pDC are involved in immunomodulation. A stable pool of regulatory T cells (Treg) is crucial for maintaining immune homeostasis. However, interactions between pDC and Treg regarding the regulation of Treg homeostasis are understudied. By using BDCA2-DTR mice as a systemic pDC depletion model, we identified increased steady-state numbers of FoxP3+ T cells with an effector Treg-like phenotype in lungs, liver, and spleen tissues. During sublethal, pulmonary Klebsiella pneumoniae infection, pDC deficiency also elevated respiratory FoxP3+ T cell numbers. Additionally, the improvement in acute pneumonia survival until day 5 post infection was accompanied by impaired proinflammatory cytokine production. In contrast, pDC-depleted mice exhibited a delayed clinical recovery during the post-acute phase. Therefore, we assume that pDC act as immunomodulators supporting the rapid onset of immune response in a proinflammatory manner and regulate inflammation or tissue regeneration in the post-acute phase. In summary, pDC assist in FoxP3+ T cell homeostasis and the regulation of Klebsiella-pneumonia progression.