Publikationsdatenbank

EHV-1 Pathogenesis: Current in vitro Models and Future Perspectives (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Kamel, Mohamed (WE 5)

Pavulraj, Selvaraj (WE 5)

Osterrieder, Klaus (WE 5)

Azab, Walid (WE 5)

Quelle

Frontiers in veterinary science : FVETS

Bandzählung: 6

Seiten: Article 251

ISSN: 2297-1769

Sprache

Englisch

Verweise

URL (Volltext): https://www.frontiersin.org/articles/10.3389/fvets.2019.00251/full

DOI: 10.3389/fvets.2019.00251

Pubmed: 31417917

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Primary infection and pathogenesis of equine herpesvirus type 1 (EHV-1) require an intricate interaction of virus with the mucosal epithelium, mononuclear cells and the vascular endothelium. Studies on EHV-1 have been facilitated by the development of different in vitro models that recapitulate the in vivo tissue complexity. The available in vitro assays can be categorized into (i) models mimicking the epithelium-peripheral blood mononuclear cell (PBMC) interaction, which include ex vivo mucosal (nasal and vaginal) explants and equine respiratory epithelial cells (EREC) cultures; and (ii) PBMC-endothelium mimicking models, including flow chamber and contact assays. These in vitro models have proven their worth in attempts to recapitulate the in vivo architecture and complexity, produce data relevant to natural host infection, and reduce animal use due to in vivo experiments. Although horse models are still needed for certain experiments, e.g., EHV-1 myeloencephalopathy or vaccination studies, available in vitro models can be used to obtain highly valuable data on virus-host tissue interactions. Microfluidic based 3D culture system (e.g., horse-on-a-chip) could be a potential upgraded version of these in vitro models for future research.