Publikationsdatenbank

Analysis of ESBL-producing Escherichia coli from pets and their owners – faecal carriage of different CTX-M-producing E. coli in a dog and a human in Portugal (2019)

Art

Poster

Autoren

Feudi, Claudia (WE 7)

Menezes, Juliana

Marques, Cátia

Belas, Adriana

Perreten, Vincent

Frosini, Siân Maria

Loeffler, Anette

Weese, Scott

Pomba, Constança

Schwarz, Stefan (WE 7)

Kongress

8th Symposium on Antimicrobial Resistance in Animals and the Environment

Tours Val de Loire - France, 01. – 03.07.2019

Quelle

8th Symposium on Antimicrobial Resistance in Animals and the Environment : Abstracts book : 1-3 July 2019 — UMR 1282 Infectiologie et Santé Publique Institut National de la Recherche Agronomique (Hrsg.)

France: INRA Science & Impact, 2019 — S. 82

Sprache

Englisch

Verweise

URL (Volltext): https://symposium.inrae.fr/arae2019/Abstract-Book

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives:
The role of companion animals as potential sources and reservoirs of antimicrobial resistant bacteria, as well as the use of antimicrobial agents crucial for human health in veterinary medicine, represent growing concerns worldwide. This study was conducted in the context of the PET-Risk Consortium, which aims to determine and analyze the bacterial transmission between pets and their owners during animal infections.

Materials and methods:
A male, 14-year old American Pit Bull terrier was admitted to the Veterinary Teaching Hospital of the Faculty of Veterinary Medicine, University of Lisbon, with a complicated urinary tract infection (UTI, bacterial cystitis). The dog had already been hospitalized in the previous 6 months and had received several courses of antimicrobials. A urine sample from the dog (by cystocentesis) and faecal samples from thedog and three household members were plated on MacConkey agar with cefotaxime (1.5 mg/L) and Superpolymyxin media. Bacterial species were identified using Maldi-TOF mass spectrometry (MS). Several PCRs were performed to determine the genetic content and relatedness of the isolates (phylogenetic groups, resistance genes and plasmid replicons). Antimicrobial susceptibility was assessed by disk diffusion tests and results interpreted according to EUCAST, except for tetracycline where CLSI recommendations wereused.

Results:
A total of twenty-seven isolates were grown and all were identified as E. coli. The isolate obtained from the infection site belonged to phylogenetic group B1, harbored a blaCTX-M-1gene and replicons I1α, FIA, FIB and FII. In contrast, E. coliisolated from the canine faecal sample belonged to phylogroup C, carried the blaCTX-M-27gene and the replicons FIA, FIB and FII. Susceptibility tests of the canine isolates revealed a multidrug resistance phenotype with resistances to third-and fourth-generation cephalosporins, fluoroquinolones, sulfamethoxazole/trimethoprim and tetracycline. Faecal E. coliisolates from two humans belonged to phylogroups A, B1, F or cryptic clade I and did not carried any blaCTX-Mgene. The third human household member harbored blaCTX-M-9-carring E. coliwhich belonged to phylogroup C and contained replicons I1α, FIB and FII.

Conclusion:
This study showed that the E. coliresponsible for the UTI was not related to the other E. coliidentified in the faecal samples, neither from the dog nor from the humans. Additionally, the E. coliisolated from animal and human feces were not related to each other and the analysis did not show any evidence of a shared colonization. To the best of our knowledge, this is the first description of canine gut colonization by a CTX-M-27–producing E. coliin Portugal. CTX-M enzymesbelonging to group 9 have rarely been reported in companion animals and the variant CTX-M-27 to an even lesser extent, as it has been described so far in cats and dogs only in Japan and UK.