Publikationsdatenbank

Isolation of Klebsiella pneumonia ST307 from a recurrent urinary tract infection of a canine patient (2019)

Art

Poster

Autoren

Bethe, Astrid (WE 7)

Eichhorn, Inga (WE 7)

Weingart, Christiane (WE 20)

Roschanski, Nicole

Schink, Anne-Kathrin (WE 7)

Brombach, Julian (WE 7)

Walther, Birgit

Kohn, Barbara (WE 20)

Lübke-Becker, Antina (WE 7)

Kongress

8th Symposium on Antimicrobial Resistance in Animals and the Environment

Tours Val de Loire - France, 01. – 03.07.2019

Quelle

8th Symposium on Antimicrobial Resistance in Animals and the Environment : Abstracts book : 1-3 July 2019 — UMR 1282 Infectiologie et Santé Publique Institut National de la Recherche Agronomique (Hrsg.)

France: INRA Science & Impact, 2019 — S. 130

Sprache

Englisch

Verweise

URL (Volltext): https://symposium.inrae.fr/arae2019/Abstract-Book

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background:
Klebsiella(K.) pneumoniaeis frequently associated with multi-drug resistant (MDR) phenotypes, including extended-spectrum beta-lactamase (ESBL)-and carbapenemase-production, and can cause a variety of infections in humans as well as in companion animals and livestock. In human medicine, the global spread of MDR K. pneumoniaeis associated with successful high-risk genetic lineages such as ST258 and the newly emerging ST307. Here, we report on multiple isolations of K. pneumoniaST307 from a dog suffering from chronic cystitis.

Material and methods:
Between December 2017 and January 2019, we investigated 19 urine samples from a 7-year-old female Sealyham terrier, suffering from a chronic urinary tract infection (UTI), during routine diagnostic. All confirmed K. pneumoniaeisolates were whole-genome sequenced (WGS) using Illumina MiSeq 300 bp paired-end sequencing with an obtained coverage of > 90x. WGS data were used for genotypic characterization including the determination of the sequence type (ST), transferable resistance genes and plasmids. In order to analyze and compare the isolates we used https://cge.dtu.dk

Results:
Twelve out of 19 urine samples were culture positive. Ten K. pneumoniaeisolates were identified in eight samples, with five being in mixed culture with E.coli, Enterococcusspp., and/or S.pseudintermedius. All K.pneumoniaebelonged to ST307, six harbored an IncF-plasmid and were phenotypically ESBL-producing. In four of these, blaCTX-M-15was identified. Phylogenetic analysis revealed a close relationship between the isolates, with a maximum of 14 SNPs detected when compared back to the first Klebsiellaisolate as reference.

Conclusion:
Although only recently genetic features that may provide anadvantage in adaption to the human host have been reported for this lineage [2, 3], MDR K.pneumoniaeST307 carrying blaCTX-M-15can cause UTI in companion animals. This is in accordance with a current study reporting on the occurrence of ST307-blaCTX-M-15in one canine and feline urine sample each [1]. Since they can serve as reservoir or source of infection for high-risk K. pneumoniaeclones, companion animals may pose a risk to their owners. Consequently, further investigation is needed to elucidate whether bacterial persistence may explain the detection of eight closely related isolates from a canine patient under antibiotic therapy in a one-year period.