Publication Database

Efficacy testing of six disinfection methods for hatching eggs against ESBL producing E.coli using eggshell samples as a carrier model (2019)

Art

Poster

Autoren

Motola, Gerzon (WE 15)

Brüggemann-Schwarze, Sarah (WE 7)

Hafez, Hafez M. (WE 15)

Kongress

Zoonoses 2019 - International Symposium on Zoonoses

Berlin, Germany, 16. – 18.10.2019

Quelle

Zoonoses 2019 - International Symposium on Zoonoses Research : Book of Abstracts — International Symposium on Zoonoses Research (Hrsg.)

Berlin, 2019 — S. 249

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/79/attachments/23/154/Book_of_Abstracts_Zoonoses2019.pdf

Kontakt

Nutztierklinik: Abteilung Geflügel

Königsweg 63
14163 Berlin
+49 30 838 62676
gefluegelkrankheiten@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction:
The presence of ESBL(extended-spectrum beta-lactamase) producing bacteria on poultry products is an important subject for veterinary and human health due to the zoonotic infection risk for producers and consumers. The present study focuses on testing the efficacy of6 different disinfection methods on hatching eggs, aiming to reduce ESBL producing contaminants at the very beginning of the production pyramid.

Materials and Methods:
Sterile eggshells cutouts wereartificially contaminated with 108ESBL producing E. coli (CTX-M-1, isolate from 1-day-old chicks) and used as carrier models for each disinfection method.The contaminated samples were separated in 2 groups; disinfected and non-disinfected. Disinfectionswere performedfollowing the products specifications. Each eggshell sample was separately crushed and bacteria re-isolated.Re-isolation rates were comparedand the disinfection efficacy determined.

Results:
The tested methods:1) Formalin gassing, 2) hydrogen peroxide-alcohol spray, 3a) essential oils spray, 4) peracetic acid foam and 5) low energetic electron radiation were able to reduce (at least 2logs) or eliminate the initial ESBL contamination.These results demonstrate the disinfection efficacy against ESBL producing E. coli, decreasing the risk of multi-resistant bacteria in one-day-old broiler chicks. 3b) Essential oils as cold fog did not reach the efficacy threshold.The five efficient methods were chosen for subsequent animal trials.