Publikationsdatenbank

Genomic characteristics of the German ESBL-producing Escherichia coli set of the JPIAMR project TransComp-ESC-R (2019)

Art

Poster

Autoren

Eichhorn, Inga (WE 7)

Trewby, Hanna

Mather, Alison E.

Wood, James

Michael, Geovana Brenner

Kaspar, Heike

Schwarz, Stefan (WE 7)

Kongress

8th Symposium on Antimicrobial Resistance in Animals and the Environment

Tours Val de Loire - France, 01. – 03.07.2019

Quelle

8th Symposium on Antimicrobial Resistance in Animals and the Environment : Abstracts book : 1-3 July 2019 — UMR 1282 Infectiologie et Santé Publique Institut National de la Recherche Agronomique (Hrsg.)

France: INRA Science & Impact, 2019 — S. 70

Sprache

Englisch

Verweise

URL (Volltext): https://symposium.inrae.fr/arae2019/Abstract-Book

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives:
For the international JPIAMR project ‘Genomic approach to transmission and compartmentalization of extended-spectrum cephalosporin resistance in Enterobacteriaceaefrom animals and humans’, short TransComp-ESC-R, Extended-Spectrum Beta-Lactamase (ESBL)-producing Escherichia coliisolates, originating from the German national resistance monitoring program GERM-Vet [1], were investigated for theirgenomic resistance characteristics.

Materials and methods:
A total of 180 ESBL-producing E. coliisolates were selected regarding the year of isolation (2008-2016) and host animal species to represent a comparable distribution towards the isolate distribution of the collaborating countries in the TransComp-ESC-Rstudy. The selection included 128 isolates from bovine, 40 from porcine, and 12 from avian sources.The 2x 300 bp paired-end sequencing was performed on the Illumina MiSeq (Illumina Inc., San Diego,USA) platform. Sequence types (STs) and resistance genes were identified with ARIBA using the ResFinder database [2, 3].

Results:
All 180 isolates harbored at least one beta lactamase-gene (median = 2), with the ESBL gene blaCTX-M-1being most common (n=120, 66.7%), followed by the broad-spectrum beta-lactamase gene blaTEM-1(n=78, 43.3%). A combination of both genes was detected in 51 isolates. Further ESBL genes identified were:blaCTX-M-14, blaCTX-M-15, blaTEM-187, blaSHV-12(2.8%), and in only three orless isolates: blaCTX-M-2, blaCTX-M-3, blaCTX-M-61, blaCTX-M-90, blaTEM-52, and blaTEM-124.The beta-lactamase genes blaOXA-1, blaOXA-320, blaTEM-57, blaTEM-206and some inhibitor-resistant TEM (IRT) beta-lactamases: blaTEM-30, blaTEM-83, and blaTEM-84 were also detected. All isolates harbored additional resistance genes to non-beta-lactam antibiotics and 174/180 (97.2%) were multidrug-resistant [with additional resistances to aminoglycoside(s), trimethoprim, colistin, chloramphenicol, fosfomycin, sulfonamide(s), streptomycin, and/or tetracycline(s)]. At least one sulfonamide resistance gene was present in 156/180 (86.6%) isolates, while the other resistance genes were detected less frequently. The isolates were assigned to 61 different STs (including threenovel STs) with ST10 as the most prominent one (24/180, 13.3%). The typical ESBL-carrying sequence types ST131 (n=1), ST167 (n=12), ST224 (n=2), ST410 (n=5) and ST617 (n=2) were represented among the isolates.

Conclusions:
Almost all ESBL-producing E. coliisolates showed multidrug-resistance. This may enable the co-selection of ESBL genes when located on the same mobile genetic element as the other resistance genes, and may influence the transmission capacity or conversely the compartmentalization of suchisolates.