Publikationsdatenbank

Expression of the tissue kallikrein-kinin cascade in granulosa cells of the ovary (2002)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Plendl, Johanna (WE 1)

Snyman, Celia

Bhoola, Kanti D

Quelle

Biological chemistry

Bandzählung: 383

Heftzählung: 12

Seiten: 1917 – 24

ISSN: 1431-6730

Sprache

Englisch

Verweise

Pubmed: 12553728

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The serine protease, tissue kininogenase (kallikrein), belongs to a unique family of enzymes that cleaves the decapeptide, kallidin, from the endogenous substrate kininogen. By analysis of genealogy patterns rat KLK gene family members have been detected in ovarian luteinizing granulosa cells of both gonadotrophin-treated and non-treated control rats. Recently, we demonstrated that tissue kininogenase showed intense immunolabeling in angiogenic endothelial cells isolated from bovine mature and regressing corpora lutea. Therefore, the question to answer was whether granulosa cells associated with ovarian vascularization possess the same capacity to express the kallikrein-kinin cascade as do microvascular endothelial cells. As a first step, experiments were designed to determine the expression and visualization of tissue kininogenase (both active and pro forms) as well as kininogen and kinin receptors in granulosa cells of different developmental stages and segments of the ovarian follicle by immunoperoxidase assay, confocal fluorescent microscopy and in situ hybridization.