Publikationsdatenbank

Murine CLCA4A and CLCA4B are Expressed in Partially Different Cellular Niches of the Intestinal Epithelium (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Teske, K. (WE 12)

Erickson, N. A. (WE 11)

Mundhenk, L. (WE 12)

Glauben, R.

Dzamukova, M.

Löhning, M.

Gruber, A. D. (WE 12)

Quelle

Journal of comparative pathology

Bandzählung: 174

Seiten: 172

ISSN: 0021-9975

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S0021997519304013

DOI: 10.1016/j.jcpa.2019.10.105

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction: The chloride channel regulator, calcium-activated 4 (CLCA4) protein is proposed as a biomarker in inflammatory bowel diseases, such as ulcerative colitis. The mouse is a common model for the human condition; however, in contrast to people, the murine Clca4 gene locus contains three duplication products, Clca4a, -4b and -4c. Their cellular expression patterns and role in diseased murine tissue are unknown to date.

Materials and Methods: The mRNA expression of the three murine Clca4 homologues were analysed by conventional or RT-qPCR in whole tissue lysates of different segments of the gastrointestinal tract of healthy C57BL/6 mice (n = 3–4). Expression levels were quantified in various compartments of the small and large intestine using laser microdissection, including intestinal villi and crypt cells. Expression of Clca4a and -4b was also compared between mice with DSS-induced colitis and healthy controls (n = 10).

Results: Clca4a and -4b were predominantly expressed in the large or small intestine of healthy mice, respectively and Clca4c was not detected at all. Additionally, Clca4a and -4b were expressed similarly in the jejunal villi and apical colonic epithelium, while Clca4b was also found in intestinal crypts. Clca4a and even more -4b, were highly overexpressed in dextran sodium sulphate-challenged mice compared with healthy controls.

Discussion: Murine Clca4a and -4b are highly expressed during inflammation, but possess partly overlapping, partly different intestinal expression patterns, which may point towards different functional roles of the two homologues in normal and inflamed tissues.