Publikationsdatenbank

Whole-genome comparative analysis of porcine MRSA ST9 and ST398 isolated in China and Germany (2019)

Art

Poster

Autoren

Ji, Xing

Feßler, Andrea T. (WE 7)

Krüger, Henrike (WE 7)

Shen, Zhangqi

Wang, Shaolin

Wang, Yang

Schwarz, Stefan (WE 7)

Wu, Congming

Kongress

8th Symposium on Antimicrobial Resistance in Animals and the Environment

Tours Val de Loire - France, 01. – 03.07.2019

Quelle

8th Symposium on Antimicrobial Resistance in Animals and the Environment : Abstracts book : 1-3 July 2019 — UMR 1282 Infectiologie et Santé Publique Institut National de la Recherche Agronomique (Hrsg.)

France: INRA Science & Impact, 2019 — S. 103

Sprache

Englisch

Verweise

URL (Volltext): https://symposium.inrae.fr/arae2019/Abstract-Book

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives:
The pig-associated methicillin-resistant Staphylococcus aureus(MRSA) sequence types ST398 and ST9 were predominant in Europe and Asia, respectively, and the reasons for this are not clear yet [1]. Differences in the accessory genome (including mobile genetic elements, MGEs) and differences in the core genome between ST9 and ST398 isolates may play an important role in the prevalence and evolution of MRSA [2].

Methods:
A total of 31 representative strains of porcine MRSA ST398 and ST9 isolated from Germany and China, were investigated by whole genome sequencing and subsequent comparisons of core and accessory genomes.

Results:
MRSA ST9 strains from China (n=10) and Germany (n=8) belong to two phylogenetic clusters, ST9-MRSA-t899-XII and ST9-MRSA-t1430-IV, respectively. Except for one Chinese ST398-MRSA-t571 strain belonging to a single clade, all ST398 strains (1 from China and 11 from Germany) belong to the cluster of ST398-MRSA-t011/t034-Vc. All Chinese ST9 (n=10) and ST398 (n=2)MRSA strains harboured aresistance gene cluster aacA-aphD-erm(C)/(T)-tet(L)-spw-lsa(E)-lnu(B)-aadDand the fexA gene. All 13 ST398 MRSA carried a unique tet(M) gene, while the eight German ST9 MRSA carried the lowest number of resistance genes. In terms of virulence genes, all 18 ST9 MRSA harboured an enterotoxin gene cluster (egc) located in the genomic island vSaβ. However, all 13 ST398 MRSA lacked the egccluster, but carried a hysAgene embedded in a truncated vSaβ. Most of the ST9 and ST398 MRSA strains harboured another genomic island vSaα, which included the von Willebrand-binding protein encoding-gene vwband the immune evasion genescn. Neither resistance genes, nor virulence genes were identified in prophages of all tested ST9 and ST398 MRSA strains. In addition, several unique chromosomal genes were found, which may be associated with colonization and adaptation, such as the cell-wall-anchored protein gene sasKonly in ST9 MRSA, or the collagen binding protein gene cna only in ST398 MRSA.

Conclusions:
ST9 MRSA in China and Germany may originate from different ancestors. The use of antibiotics may have played an important role in the development and selection of MRSA. In addition to differences in MGEs, differences in chromosomal genes are likely to play a central role in the pathogenicity and adaptability of MRSA.