Publikationsdatenbank

Comparison of antimicrobial resistance genes in LA-MRSA CC398 from food-producing animals in Germany (2019)

Art

Poster

Autoren

Krüger, Henrike (WE 7)

Jiang, Nansong

Ji, Xing

Feßler, Andrea T. (WE 7)

Kadlec, Kristina

Shen, Zhangqi

Wang, Shaolin

Ehricht, Ralf

Monecke, Stefan

Wang, Yang

Wu, Congming

Schwarz, Stefan (WE 7)

Kongress

8th Symposium on Antimicrobial Resistance in Animals and the Environment

Tours Val de Loire - France, 01. – 03.07.2019

Quelle

8th Symposium on Antimicrobial Resistance in Animals and the Environment : Abstracts book : 1-3 July 2019 — UMR 1282 Infectiologie et Santé Publique Institut National de la Recherche Agronomique (Hrsg.)

France: INRA Science & Impact, 2019 — S. 71

Sprache

Englisch

Verweise

URL (Volltext): https://symposium.inrae.fr/arae2019/Abstract-Book

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Aims:
In this study, we compared the antimicrobial resistance genes present in livestock-associated methicillin-resistant Staphylococcus aureus(LA-MRSA) of the clonal complex CC398 obtained from pigs, cattle, or poultry in Germany.

Methods:
A total of 95 epidemiologically unrelated LA-MRSA ST398 collected from pigs (n = 54), cattle (n = 25), turkeys (n = 11) and chickens (n = 5) were analyzed for their antimicrobial resistance genes. The resistance genes were detected by a S. aureus-specific DNA microarray, PCR and/or sequence analysis.Their functionality was confirmed by MIC testing.

Results:
LA-MRSA from all origins carried the β-lactam resistance gene mecAand 94/95 isolates also harbored the blaZ/I/Roperon. Among the tetracycline resistance genes, tet(M) was predominantly found, followed by tet(K) and tet(L). The macrolide-lincosamide-streptogramin B resistance gene erm(C) was most frequently detected, followed by erm(B), erm(T) and erm(A). The predominant aminoglycoside resistance gene was aacA-aphD, followed by aadDand aphA3. The most frequently seen aminocyclitol resistance gene was spc. Among the trimethoprim resistance genes, dfrKwas most commonly observed although some isolates from pigs harbored dfrG. The phenicolresistance gene fexA, the apramycin resistance gene apmA, the lincosamide resistance gene lnu(A) and the pleuromutilin-lincosamide-streptogramin A resistance genes vga(A), vga(C), and vga(E) were only rarely detected. None of the isolates carried the multiresistance gene cfr. Only minor host species-specific variations in the resistance gene content were observed.

Conclusions:
A wide variety of antimicrobial resistance genes was detected among LA-MRSA from food-producing animals in Germany. The same genes were detected in isolates from different hosts. Occasionally more than one resistance gene conferring the same resistance phenotype was detected, e.g. tet(M) + tet(K) + tet(L). Genes conferring resistance to last resort antimicrobial agents in human medicine, such as the oxazolidinones, were not detected among the strains tested.