Publikationsdatenbank

Characterization of blaCTX-M-1 gene regions on plasmids from Escherichia coliisolates collected in the GERM-Vet resistance monitoring program 2008-2015 fromdiseased food-producing animals (2019)

Art

Poster

Autoren

Schink, A.-K. (WE 7)

Michael, G. B.

Hanke, D. (WE 7)

Kaspar, H.

Falgenhauer, L.

Imirzalioglu, C.

Chakraborty, T.

Schwarz, S. (WE 7)

Kongress

Zoonoses 2019 - International Symposium on Zoonoses Research

Berlin, 16. – 18.10.2019

Quelle

Zoonoses 2019 - International Symposium on Zoonoses Research : Book of Abstracts — International Symposium on Zoonoses Research (Hrsg.)

Berlin, 2019 — S. 242

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/79/attachments/23/154/Book_of_Abstracts_Zoonoses2019.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives:
The aim of the study was to analyse the genetic environment of plasmid-borne blaCTX-M-1 genes ofEscherichia coli isolates from diseased food-producing animals.

Materials and methods:
The ESBL gene blaCTX-M-1 was identified in 352 of 7,810 E. coli isolates from diseased food-producing animals collected in GERM-Vet (2008-2015).Fifty representative isolates were subjected to plasmid transfer experiments and whole genome sequencing. The transformed plasmids were characterized by PCR-based replicon typing and PCR assays for the detection of co-located resistance genes.

Results:
The plasmids were positive for nine different replicons or replicon combinations, one plasmid was non-typeable. ISEcp1, bracketed by 5-bp direct repeats,was associated with blaCTX-M-1 on most IncI1 plasmids. On IncN, IncFII and most IncF plasmids ISEcp1 was truncated by IS26 in the upstream region and downstream a Δmrx-mph(A) cluster was detected followed by IS26at varying positions in the sequences of different plasmids. Commonly detected co-located antimicrobial resistance genes conferred resistance to sulphonamides, aminoglycosides and tetracycline.

Conclusions:
The genetic environment of the blaCTX-M-1 genes was diverse, but similarities among the different plasmid families were noted. IncN, IncI1 and IncF plasmids play a role in the dissemination of blaCTX-M-1;the co-located antimicrobial resistance genes may facilitate the spread and persistence of this ESBL gene.