Publication Database

Analysis of the genomic environment of plasmid-borne bla_CTX-M-1 genes from Escherichia coli isolates of diseased food-producing animals collected in GERM-Vet 2008-2015 (2019)

Art

Poster

Autoren

Schink, Anne-Kathrin (WE 7)

Michael, Geovanna Brenner

Hanke, Dennis (WE 7)

Kaspar, Heike

Falgenhauer, Linda

Imirzalioglu, Can

Chakroborty, Trinad

Schwarz, Stefan (WE 7)

Kongress

8th Symposium on Antimicrobial Resistance in Animals and the Environment

Tours Val de Loire - France, 01. – 03.07.2019

Quelle

8th Symposium on Antimicrobial Resistance in Animals and the Environment : Abstracts book : 1-3 July 2019 — UMR 1282 Infectiologie et Santé Publique Institut National de la Recherche Agronomique (Hrsg.)

France: INRA Science & Impact, 2019 — S. 101

Sprache

Englisch

Verweise

URL (Volltext): https://symposium.inrae.fr/arae2019/Abstract-Book

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives:
The predominant extended-spectrum β-lactamase (ESBL) gene among German Escherichia coliisolates from diseased animals is blaCTX-M-1 [1]. This study focused on the sequence analysis of blaCTX-M-1 gene regions found on different plasmid types of E. colioriginating from diseased food-producing animals.

Materials and methods:
The German national resistance monitoring program GERM-Vet collected a total of 7,810 E. coliisolates from diseased cattle (n=3,188), pigs (n=1,834) and poultry (n=2,788) between 2008 and 2015. In 352 E. coliisolates (cattle n=253, pigs n=84, poultry n=15) the presence of the gene blaCTX-M-1 was confirmed by PCR and sequencing [2]. A subset of 49 isolates was subjected to plasmid transfer experiments and whole genome sequencing using the Illumina MiSeq platform. The transformed plasmids were characterized by replicon typing, antimicrobial susceptibility testing of the transformants and PCR assays for the detection of co-located resistance genes.

Results:
The 49 plasmids belonged to incompatibility groups IncN (n=19), IncI1 (n=13), IncFII (n=5), IncF (n=5), IncB/O (n=3) and IncY (n=1), two were positive for the replicons FII and FIB or I1 and FIA, respectively, and one plasmid was non-typeable. Onthe IncN, IncFII, and most IncF plasmids, as well as the IncY and the non-typeable plasmid, an ISEcp1, truncated by IS26, was detected upstream of blaCTX-M-1. Downstream of blaCTX-M-1,a Δmrx-mph(A) cluster was identified, followed by IS26at 45 bp, 21 bpor 3 bp downstream of mph(A).Differently, on one plasmid the IS26truncated the mph(A) gene. On most of the IncI1 plasmids, blaCTX-M-1 was associated with ISEcp1bracketed by direct repeats AAAAA or TTATA, respectively. On one IncI1 plasmid ISEcp1was not flanked by direct repeats and on another an IS element belonging to the IS5family was detected downstream of blaCTX-M-1. A complete ISEcp1was detected on one IncB/O plasmid; on the other two plasmids, a fragment of ISEcp1was identified upstream of blaCTX-M-1while downstream Δmrx-mph(A) clusters were identified of which one mph(A) gene was truncated by IS26. Twenty-seven plasmids carried no co-located resistance genes and belonged mainly to IncN (n=18). Commonly detected co-located resistance genes were sul2(n=13), aac(3)-IVa(n=8) and tet(A) (n=4), which confer resistance to sulfonamides, apramycin/gentamicin or tetracycline, respectively.

Conclusions:
IncN, IncI1 and IncF plasmids play an important role in the dissemination of blaCTX-M-1 genes among E. colifrom diseased animals in Germany. The blaCTX-M-1 gene regions were diverse, but similar among the different plasmid families. Moreover, the co-located resistance genes may facilitate the spread and persistence of blaCTX-M-1-carrying plasmids.