Publikationsdatenbank

Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Loncaric, Igor

Kübber-Heiss, Anna

Posautz, Annika

Ruppitsch, Werner

Lepuschitz, Sarah

Schauer, Bernhard

Feßler, Andrea T (WE 7)

Krametter-Frötscher, Reinhild

Harrison, Ewan M

Holmes, Mark A

Künzel, Frank

Szostak, Michael P

Hauschild, Tomasz

Desvars-Larrive, Amélie

Misic, Dusan

Rosengarten, Renate

Walzer, Chris

Slickers, Peter

Monecke, Stefan

Ehricht, Ralf

Schwarz, Stefan (WE 7)

Spergser, Joachim

Quelle

Veterinary microbiology : an international journal

Bandzählung: 230

Seiten: 138 – 144

ISSN: 0378-1135

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S0378113518314792?via%3Dihub

DOI: 10.1016/j.vetmic.2019.02.014

Pubmed: 30827379

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecR1-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.