Publikationsdatenbank

Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Khan, Aman Ullah

Melzer, Falk

El-Soally, Sherif Abdel Ghafar Elsayed

Elschner, Mandy C.

Mohamed, Shereen Aziz

Sayed Ahmed, Mohamed Abdelmonem

Roesler, Uwe (WE 10)

Neubauer, Heinrich

El-Adawy, Hosny

Quelle

Pathogens

Bandzählung: 8

Heftzählung: 4

Seiten: Article 248

ISSN: 2076-0817

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2076-0817/8/4/248

DOI: 10.3390/pathogens8040248

Pubmed: 31756893

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Brucellosis is considered as endemic disease of animals and humans since thousands of years in Egypt. However, brucellosis in pigs has never been reported in Egypt. Thus, serological and molecular assays were applied to detect anti-Brucella antibodies and DNA in serum samples collected from pigs. In total 331 blood samples collected from male and female pigs at slaughterhouses of Cairo and Giza governorates were investigated using Brucella c- and i-ELISA and Brucella real-time PCR. Anti-Brucella antibodies were detected in 16 (4.83%) and 36 (10.8%) sera by i-ELISA and c-ELISA, respectively. Brucella DNA was detected in 10 (3.02%) seropositive samples and identified as Brucellamelitensis (7/10) andBrucellasuis (3/10). A higher prevelance was found in boars. This is the first study investigating pig brucellosis in Egypt. The results of this study will raise awareness for brucellosis in these farm animals and will help to develop effective control strategies.