Publikationsdatenbank

Efficacy of six different disinfection methods against artificial ESBL producting E. coli contamination on broiler hatching eggs and effects on hatchability (2019)

Art

Poster

Autoren

Motola, Gerzon (WE 15)

Tebrün, Wiebke

Pees, Michael

Bachmeier, Josef

Hafez, Hafez Hafez (WE 15)

Brüggemann-Schwarze, Sarah (WE 15)

Kongress

FEMS Congress

Glasgow, Scotland, 07. – 11.07.2019

Quelle

FEMS 2019 : 8th Congress of European Microbiologists : abstract book — Congress of European Microbiologists, in collaboration with sfam (Society for Applied Microbiology) (Hrsg.)

Glasgow, Scotland, 2019 — S. 1477

Sprache

Englisch

Verweise

URL (Volltext): https://fems2019.org/fileadmin/user_upload/FEMS/fems2019_abstractbook.pdf

Kontakt

Nutztierklinik: Abteilung Geflügel

Königsweg 63
14163 Berlin
+49 30 838 62676
gefluegelkrankheiten@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background:
ESBL (extended-spectrum beta-lactamase) producing bacteria in poultry products are of major concern for the poultry industry and consumers. The present study focusses on the reduction of ESBL producing E. coli at the beginning of the broiler production chain to avoid a vertical transmission.

Objectives:
This study focusses on testing the efficacy of six different disinfection methods against ESBL producing bacteria in broiler hatching eggs before incubation, and their effect on the hatchability.

Methods:
The study is divided into two segments: Disinfection efficacy on eggshell samples and effect on hatchability. 20 sterile eggshells cutouts were used as carriers and artificially contaminated with 108 ESBL producing E. coli (strain 10682 CTX-M-1, phylogroup B1). Half of the samples were disinfected. Afterwards, cfu were determined for each and reisolation rates compared between disinfected and non-disinfected.
For the hatchability test, three groups were created with 50 broiler hatching eggs each; contaminated and not disinfected, contaminated and disinfected and neither contaminated nordisinfected. Contamination of the eggs was fulfilled using temperature difference egg-dipping in cold bacterial suspension. All groups were separated during the study. After 21 days of egg incubation, the hatching rate was determined.

Results:
The six tested methods, namely Formalin fumigation, H2O2 + alcohol, essential oils spray and cold fog, peracetic acid and low energy electron beam, reduced the artificial contamination on the egg shell samples. Five of six methods had no negative effect on hatchability. Essential oils as spray
reduced significantly the hatchability and will not be used for following field trials.