Publikationsdatenbank

Staphylococcus aureus from zoo and wildlife animals (2018)

Art

Poster

Autoren

Feßler, Andrea (WE 7)

Thomas, Patricia

Grobbel, Mirjam

Brombach, Julian (WE 7)

Eichhorn, Inga (WE 7)

Monecke, Stefan

Ehricht, Ralf

Schwarz, Stefan

Kongress

18th International Symposium on Staphylococci and Staphylococcal Infections

Copenhagen, Denmark, 23. – 26.08.2018

Quelle

International Symposium on Staphylococci and Staphylococcal Infections — International Symposium on Staphylococci and Staphylococcal Infections (Hrsg.)

Copenhagen, Denmark, 2018 — S. 397

Sprache

Englisch

Verweise

URL (Volltext): http://isssi2018.org/fileadmin/user_upload/ISSI2018/ISSSI2018_programme_abstract_book_web5.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Aims:
As there is comparatively little information available about Staphylococcus aureus isolates from zoo and wildlife animals, we investigated such isolates for their pheno- and genotypes.

Methods:
Twenty-three S. aureus isolates (nine from wild animals and 14 from zoo animals) were obtained during routine diagnostics. All isolates were characterized by multilocus sequence typing (MLST), spa typing, macrorestriction analysis with subsequent SmaI pulsed-field gelelectrophoresis (PFGE), antimicrobial susceptibility testing and S. aureus-specific DNA-microarray analysis. Antimicrobial resistance genes were identified by PCR.

Results:
Isolates from zoo animals and wildlife differed distinctly in their MLST types, spa types and PFGE patterns. Nineteen spa types were identified, including three novel types and 16 main macrorestriction patterns were seen. Only few isolates were resistant and the genes conferring resistance to β-lactams [blaZ,
mecA, mecC], tetracyclines [tet(K), tet(L)] and chloramphenicol [cat pC221]) or fluoroquinolone resistance-mediating mutations (GyrA: Ser84Leu, GyrB: Leu469Val, GrlA: Ser80Phe) were detected. DNA microarray analysis revealed that all isolates were negative for Panton-Valentine leukocidin (PVL) genes, but the animal-associated leukocidin genes lukM/lukF-P83 were present in three isolates from two animals. Five S. aureus isolates harboured several enterotoxin genes, whereas the toxic shock syndrome toxin gene tst1 was only present in one zoo animal isolate.

Discussion:
S. aureus from zoo and wild animals showed a high diversity in their genotypic properties. The presence of mecC in an isolate from a flying fox or tst1 in an isolate from a Mongolian sheep raise questions on how these animals have acquired the respective S. aureus isolates.