Publikationsdatenbank

Contribution of the Cpx envelope stress system to metabolism and virulence regulation in Salmonella enterica serovar Typhimurium (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Subramaniam, Sivaraman

Müller, Volker S.

Hering, Nina A.

Mollenkopf, Hans

Becker, Daniel

Heroven, Ann Kathrin

Dersch, Petra

Pohlmann, Anne

Tedin, Karsten (WE 7)

Porwollik, Steffen

McClelland, Michael

Meyer, Thomas F.

Hunke, Sabine

Quelle

PLOS ONE

Bandzählung: 14

Heftzählung: 2

Seiten: e0213297

ISSN: 1932-6203

Sprache

Englisch

Verweise

URL (Volltext): https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0211584

DOI: 10.1371/journal.pone.0211584

Pubmed: 30716090

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The Cpx-envelope stress system regulates the expression of virulence factors in many Gram-negative pathogens. In Salmonella enterica serovar Typhimurium deletion of the sensor kinase CpxA but not of the response regulator CpxR results in the down regulation of the key regulator for invasion, HilA encoded by the Salmonella pathogenicity island 1 (SPI-1). Here, we provide evidence that cpxA deletion interferes with dephosphorylation of CpxR resulting in increased levels of active CpxR and consequently in misregulation of target genes. 14 potential operons were identified to be under direct control of CpxR. These include the virulence determinants ecotin, the omptin PgtE, and the SPI-2 regulator SsrB. The Tat-system and the PocR regulator that together promote anaerobic respiration of tetrathionate on 1,2-propanediol are also under direct CpxR control. Notably, 1,2-propanediol represses hilA expression. Thus, our work demonstrates for the first time the involvement of the Cpx system in a complex network mediating metabolism and virulence function.