Publikationsdatenbank

MR Angiography of the Head/Neck Vascular System in Mice on a Clinical MRI System (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Reimann, Carolin

Brangsch, Julia

Adams, Lisa Christine

Thöne-Reineke, Christa (WE 11)

Hamm, Bernd

Makowski, Marcus Richard

Quelle

Contrast media & molecular imaging

Bandzählung: 2019

Seiten: Article ID 5461809

ISSN: 1555-4317

Sprache

Englisch

Verweise

URL (Volltext): https://www.hindawi.com/journals/cmmi/2019/5461809/

DOI: 10.1155/2019/5461809

Pubmed: 31275084

Kontakt

Institut für Tierschutz, Tierverhalten und Versuchstierkunde

Königsweg 67
14163 Berlin
+49 30 838 61146
tierschutz@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Magnetic resonance angiography (MRA) represents a clinical reference standard for the in vivo assessment of the vasculature. In this study, the potential of non-contrast-enhanced and contrast-enhanced angiography of the head/neck vasculature in mice on a clinical MR imaging system was tested.

All in vivo magnetic resonance imaging was performed with a 3T clinical system (Siemens). Non-contrast-enhanced (time-of-flight, TOF) and contrast-enhanced angiography (gadofosveset-trisodium, GdT) were performed in C57BL/6J mouse strain. Lumen-to-muscle ratios (LMRs) and area measurements were assessed. Histology was performed as reference standard of all relevant vascular structures.

A close correlation between TOF (R2 = 0.79; p < 0.05) and contrast-enhanced (GdT) angiography (R2 = 0.92; p < 0.05) with histological area measurements was found. LMRs were comparable between both sequences. Regarding interobserver reproducibility, contrast-enhanced (GdT) angiography yielded a smaller 95% confidence interval and a closer interreader correlation compared to non-contrast-enhanced (TOF) measurements (-0.73-0.89; R2 = 0.81 vs. -0.55-0.56; R2 = 0.94).

This study demonstrates that non-contrast-enhanced and contrast-enhanced angiographies of the head/neck vasculature of small animals can reliably performed on a clinical 3T MR scanner. Contrast-enhanced angiography enables the visualization of vascular structures with higher intravascular contrast and higher reproducibility.