Publikationsdatenbank

Storage procedures and time influence the detectability of Clostridium difficile toxin A but not toxin B in porcine fecal specimens (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Grześkowiak, Łukasz (WE 4)

Riedmüller, Jonathan (WE 4)

Vahjen, Wilfried (WE 4)

Zentek, Jürgen (WE 4)

Quelle

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians

Seiten: 1040638719864383

ISSN: 1040-6387

Sprache

Englisch

Verweise

URL (Volltext): https://journals.sagepub.com/doi/10.1177/1040638719864383#abstract

DOI: 10.1177/1040638719864383

Pubmed: 31328697

Kontakt

Institut für Tierernährung

Königin-Luise-Str. 49
14195 Berlin
+49 30 838 52256
tierernaehrung@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Storage procedures are known to affect the detectability of Clostridium difficile toxins in equine and human feces. We assessed the impact of different storage conditions on the detectability of C. difficile toxins in swine feces. Specimens were inoculated with toxins, 112 ng/g of toxin A (TcdA) and 16 ng/g of toxin B (TcdB) and subjected to the following 3 storage treatments: 4°C, -30°C, repetitive freezing at -30°C and thawing. Toxin determination was assessed at 1, 2, 7, 14, and 21 d with ELISA. A decrease in concentrations of TcdA with time was observed for samples stored at 4°C and repetitive freezing-thawing (p ≤0.05). On day 14, storage at 4°C resulted in decreased TcdA concentration as opposed to storage at -30°C and repetitive freezing-thawing (p ≤0.05). On day 21, storage at 4°C resulted in decreased TcdA detectability compared with storage at -30°C (p ≤0.05). The TcdB concentration was unaffected. These results on toxin detectability in swine feces should be carefully considered in in vitro studies on toxigenic C. difficile. Our results also offer valuable information for microbiologists and veterinarians monitoring the presence of virulent C. difficile in pigs.