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Temperature monitoring during critical care provides important data required to guide treatment delivery. Body temperature
is an easily quantified clinical parameter that can yield much information concerning the health of an animal. In research
settings, temperature has been adopted as a means to judge humane endpoints. Therefore, reliable, noninvasive, and inexpen-
sive methods for temperature monitoring are becoming a necessity in research laboratories. This study aimed to determine
the accuracy and agreement of using an infrared camera as an alternative method of temperature measurement in mice and
to compare the accuracy of this noninvasive method with established subcutaneous, intraperitoneal, and rectal techniques.
Measurement of body-surface temperature by using an infrared camera was compared with these 3 established methods in
male NMRI nude mice (
n
= 10; age, 10 mo); data were obtained 3 times daily over 14 d. Subcutaneous temperatures were
measured remotely by using a previously implanted subcutaneous temperature transponder, after which temperature was
measured by using noncontact infrared thermometry and a rectal probe. Measurements from intraperitoneal data loggers were
obtained retrospectively. The data show that using an infrared camera provides a simple, reliable method for measuring body
temperature in male NMRI nu/nu mice that minimizes handling and is minimally invasive. Whether infrared thermometry
is a useful method for measuring body temperature in furred mice warrants further investigation.