Publikationsdatenbank

Effects of bilateral hydrostatic pressure incubation on the barrier function of mammary epithelial cells (2018)

Art

Poster

Autoren

Mießler, K (WE 2)

Markov, Alexander G (WE 2)

Amasheh, Salah (WE 2)

Kongress

23. Tagung der DVG-Fachgruppe Physiologie und Biochemie der Deutschen Veterinärmedizinischen Gesellschaft

Wien, 21. – 23.02.2018

Quelle

23. Tagung der Fachgruppe Physiologie und Biochemie der Deutschen Veterinärmedizinischen Gesellschaft PROGRAMM & ABSTRACTS — Veterinärmedizinische Universität Wien (Hrsg.)

Wien, 2018 — S. P10

Verweise

URL (Volltext): https://www.vetmeduni.ac.at/fileadmin/v/DVG-Tagung-2018/160218_Abstract_Program_Guide_sortiert_final.pdf

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Outline:
A paracellular sealing effect was observed during milk accumulation in the murine mammary gland, reflected by a changed expression of tight junction proteins in the alveolar epithelial cells (1). To differentiate between effects of hydrostatic pressure and other known influencing factors like milk secretion (2), a modified Ussing chamber had been established to analyze effects of hydrostatic pressure, in vitro (3). Using this tool, we tested bilateral hydrostatic pressure effects in dependence of BaCl2 on barrier properties and tight junction proteins in a mammary epithelial cell model.

Methods:
Monolayers of the mammary epithelial cell line HC11 were grown on permeable supports for 7 d, and mounted in modified Ussing chambers. Cells were incubated with a bilateral pressure of 10 kPa for 4 hours. In a parallel approach, 1 mM BaCl2 was added to the bathing solution. Short circuit current (ISC) and transepithelial resistance (RT) were recorded during incubation, and analyzed compared to controls. After pressure incubation, western blotting and quantification was performed for the tight junction proteins occludin, claudin-3, claudin-4 and ZO-1.

Results:
In both approaches, ISC decreased during bilateral hydrostatic pressure incubation whereas no significant changes were observed for RT, compared to controls. On molecular level, a reduction claudin-3 and claudin-4 was observed in the setup without BaCl2, compared to controls. Interestingly, with addition of BaCl2 to the bathing solution changes in tight junction composition could not be observed.

Conclusions:
The reduction of ISC and the changes in claudin-3 and claudin-4 could represent cellular adaptive mechanisms. However, the reduction of sealing tight junction proteins may indicate that further maturing processes are needed for HC11 cells to form a physiological barrier, like a longer cultivation time or hormone-induced differentiation.