Publikationsdatenbank

Intracellular free magnesium concentration in healthy horses (2018)

Art

Vortrag

Autoren

Winter, Judith (WE 17)

Sponder, G. (WE 2)

Merle, Roswitha (WE 16)

Aschenbach, J.R. (WE 2)

Gehlen, H. (WE 17)

Kongress

DVG Vet Congress Berlin

Berlin, 05. – 06.10.2018

Quelle

Journal of animal physiology and animal nutrition : official journal of the European Society of Veterinary and Comparative Nutrition and the American Academy of Veterinary Nutrition

Bandzählung: 102

Heftzählung: 5

Seiten: 1351 – 1356

ISSN: 0931-2439

Sprache

Englisch

Verweise

DOI: 10.1111/jpn.12921

Kontakt

Institut für Veterinär-Epidemiologie und Biometrie

Königsweg 67
14163 Berlin
+49 30 838 56034
epi@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Equine metabolic syndrome (EMS) is a worldwide disease in horses that parallels human diabetes mellitus type 2. In both diseases, patients show an altered peripheral insulin sensitivity as a key feature. In humans, multiple studies have demonstrated the beneficial effect of magnesium supplementation on insulin sensitivity. However, serum magnesium levels vary and are therefore not a reliable indicator of the patients' magnesium status. Determining the intracellular free magnesium concentration appears to be a more sensitive diagnostic indicator. In this study, the free intracellular magnesium concentration was measured using mag-fura 2 spectrophotometry in blood lymphocytes in 12 healthy, non-obese horses at 9 a.m., 12 a.m. and 4 p.m. to establish reference ranges according to a protocol designed for human blood lymphocytes. Additionally, the serum magnesium concentration was measured. In all horses, the total serum magnesium concentration was within the reference range. The mean free magnesium concentration in blood lymphocytes of all horses was 0.291 ± 0.067 mmol/L with no significant difference between the time points. The reference range for the free intracellular magnesium concentration in equine lymphocytes was set at 0.16-0.42 mmol/L. The established values are slightly lower than those in healthy humans. The designed protocol for the measurement of the intracellular free magnesium concentration might be an excellent research tool to assess the cellular magnesium status and to reliably diagnose an altered magnesium homeostasis in EMS. Further studies shall elucidate possible alterations in cellular magnesium status in horses with EMS.