Publikationsdatenbank

Development to term of sheep embryos reconstructed after inner cell mass/trophoblast exchange (2018)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Loi, Pasqualino

Galli, Cesare

Lazzari, Giovanna

Matsukawa, Kazutsugu

Fulka, Josef

Goeritz, Frank

Hildebrandt, Thomas B (WE 19)

Quelle

The Journal of reproduction and development

Bandzählung: 64

Heftzählung: 2

Seiten: 187 – 191

ISSN: 0916-8818

Sprache

Englisch

Verweise

DOI: 10.1262/jrd.2017-109

Pubmed: 29445070

Kontakt

Tierklinik für Fortpflanzung

Königsweg 65
Haus 27
14163 Berlin
+49 30 838 62618
fortpflanzungsklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Here we report in vitro and term development of sheep embryos after the inner cell mass (ICM) from one set of sheep blastocysts were injected into the trophoblast vesicles of another set. We also observed successful in vitro development of chimeric blastocysts made from sheep trophoblast vesicles injected with bovine ICM. First, we dissected ICMs from 35 sheep blastocysts using a stainless steel microblade and injected them into 29 re-expanded sheep trophoblastic vesicles. Of the 25 successfully micromanipulated trophoblastic vesicles, 15 (51.7%) re-expanded normally and showed proper ICM integration. The seven most well reconstructed embryos were transferred for development to term. Three ewes receiving manipulated blastocysts were pregnant at day 45 (42.8%), and all delivered normal offspring (singletons, two females and one male, average weight: 3.54 ± 0.358 kg). Next, we monitored in vitro development of sheep trophoblasts injected with bovine ICMs. Of 17 injected trophoblastic vesicles, 10 (58.8%) re-expanded after 4 h in culture, and four (40%) exhibited integrated bovine ICM. Our results indicate that ICM/trophoblast exchange is feasible, allowing full term development with satisfactory lambing rate. Therefore, ICM exchange is a promising approach for endangered species conservation.