Publikationsdatenbank

Overexpression of cellular telomerase RNA enhances virus-induced cancer formation (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Kheimar, Ahmed (WE 5)

Trimpert, Jakob (WE 5)

Groenke, Nicole (WE 5)

Kaufer, Benedikt B (WE 5)

Quelle

Oncogene

Bandzählung: 38

Heftzählung: 10

Seiten: 1778 – 1786

ISSN: 1476-5594

Sprache

Englisch

Verweise

DOI: 10.1038/s41388-018-0544-1

Pubmed: 30846849

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The telomerase RNA subunit (TR) is overexpressed in many tumors; however, the contribution of TR in cancer formation remains elusive. The most frequent clinically diagnosed cancer in the animal kingdom is caused by the highly oncogenic herpesvirus Marek's disease virus (MDV). MDV encodes a TR (vTR) that plays an important role in virus-induced tumorigenesis and shares 88% sequence identity with its cellular homologue. To determine if the cellular TR possesses pro-oncogenic activity, we replaced vTR with the cellular homologue in the virus genome. Insertion of cellular TR resulted in a strong overexpression in virus infected cells, while virus replication was not affected. Strikingly, cellular TR promoted tumor formation as efficient as vTR, while tumorigenesis was severely impaired in the absence of vTR. Our data provide the first evidence that overexpression of cellular TR can contribute to tumor formation in vivo using this natural virus-host model for herpesvirus-induced oncogenesis.