Publikationsdatenbank

Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Barton, Ann Kristin (WE 17)

Shety, Tarek (WE 17)

Klier, John

Geis, Sabine (WE 17)

Einspanier, R (WE 3)

Gehlen, H. (WE 17)

Quelle

Mediators of inflammation

Bandzählung: 2019

Seiten: Article ID 7845623

ISSN: 0962-9351

Verweise

URL (Volltext): https://www.hindawi.com/journals/mi/2019/7845623/

DOI: 10.1155/2019/7845623

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Objectives. Inhalation of immunostimulatory bacterial DNA segments (cytosine-phosphate-guanosine-oligodeoxynucleotides,
CpG-ODN) normalizes clinical and cytologic parameters in severe equine asthma. We hypothesized that CpG-ODN inhalation
also reduces the misbalance of elastinolytic activity in asthmatic horses. Methods. Twenty asthmatic horses diagnosed by clinical
examinations using a scoring system were included. All horses inhaled CpG-ODNs for 14 days in 2-day intervals. Matrix
metalloproteinase (MMP-2/-9) and tissue inhibitors of metalloproteinase (TIMP-1/-2) concentrations were measured in tracheal
aspirates using equine sandwich ELISAs before and 2 and 6 weeks after CpG-ODN inhalation. Results. MMP and TIMP
concentrations correlated with the results of clinical scoring in all stages of equine asthma. Inhalation therapy led to significant
reductions in clinical scores. MMP-2, MMP-9, and TIMP-2 concentrations were significantly reduced immediately, and all
MMP and TIMP concentrations 6 weeks after therapy. Discussion. In equine asthma, overexpression of MMPs contributes to
pathological tissue destruction, while TIMPs counteract MMPs with overexpression leading to fibrosis formation. The results
of this study show that CpG-ODN inhalation may be an effective therapy to address a misbalance in equine asthma.
Conclusions. Misbalance of elastinolytic activity seems to improve by CpG-ODN inhalation for at least 6 weeks posttherapy,
which may reduce the remodeling of the extracellular matrix. Further studies should evaluate this effect in comparison to
glucocorticoid inhalation therapy. Significance. CpG-ODN inhalation may be an effective therapy in the prevention of
pulmonary fibrosis formation in equine asthma.