Publikationsdatenbank

Serotypes and antimicrobial resistance patterns of Salmonella enterica subsp. enterica in pork and related fresh vegetable servings among pork outlets in Kampala, Uganda (2018)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Ndoboli, D.

Roesel, K. (WE 13)

Heilmann, M. (WE 13)

Alter, T. (WE 8)

Clausen, P.H. (WE 13)

Wampande, E.

Grace, D.

Huehn, S. (WE 8)

Quelle

Revue d’élevage et de médecine vétérinaire des pays tropicaux

Bandzählung: 71

Heftzählung: 1-2

Seiten: 31289

ISSN: 1951-6711

Sprache

Englisch

Verweise

URL (Volltext): http://revues.cirad.fr/index.php/REMVT/article/view/31289

DOI: 10.19182/remvt.31289

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The aim of this study was to characterize serotypes, phenotypic antimicrobial resistance patterns, and plasmid profiles of 55 Salmonella enterica subsp. enterica isolates in different matrices from 77 pork outlets in Kampala, Uganda. Seven different serovars were identified, namely Enteritidis (60%), Offa (10.9%), Gallinarum (7.3%), Arechavaleta (monophasic) (7.3%), Zanzibar (7.3%), Kampala (5.4%), and Saintpaul (1.8%). Most isolates were obtained from raw pork (40.0%), followed by flies (27.3%), raw vegetables (18.2%), water (12.7%), and roasted pork (1.8%). All but one of the isolates (98%) showed resistance to at least one of the 22 antimicrobials tested, with highest levels of resistance expressed to cefazolin (95%), and cefotaxime (93%). Intermediate resistance was found to ciprofloxacin (58%), chloramphenicol (58%), and amoxicillin-clavulanic acid (56%). Most isolates were susceptible to levofloxacin (75%), sulfamethoxazole-trimethoprim (80%), and ofloxacin (96%). Characterization of strains by PCR-based replicon typing detected the presence of FIA, FIB, FIC, HI1, HI2, I1-1ᵞ, L/M, N, P, W, T, A/C, K, B/O, X, Y, F and FIIA replicons. Six replicon groups (FIA, W, FIC, FIB, P, and Y) were identified in 53 of the 55 (96.4%) isolates with more than one group existing among 42 different isolates. Although the average number of replicon groups per strain was low (2.6), phenotypical resistance rates remained high implying that some strains seemed to encode resistances on the chromosome or undetected plasmids, respectively. Potential drivers in livestock production and human medicine, and sources of antimicrobial resistance need to be identified to protect public health in Uganda.