Publikationsdatenbank

The olfactory epithelium as a port of entry in neonatal neurolisteriosis (2018)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Pägelow, Dennis (WE 7)

Chhatbar, Chintan

Beineke, Andreas

Liu, Xiaokun

Nerlich, Andreas

van Vorst, Kira (WE 7)

Rohde, Manfred

Kalinke, Ulrich

Förster, Reinhold

Halle, Stephan

Valentin-Weigand, Peter

Hornef, Mathias W

Fulde, Marcus (WE 7)

Quelle

Nature Communications

Bandzählung: 9

Heftzählung: 1

Seiten: 1 – 13

ISSN: 2041-1723

Sprache

Englisch

Verweise

DOI: 10.1038/s41467-018-06668-2

Pubmed: 30323282

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Bacterial infections of the central nervous system (CNS) remain a major cause of mortality in the neonatal population. Commonly used parenteral infection models, however, do not reflect the early course of the disease leaving this critical step of the pathogenesis largely unexplored. Here, we analyzed nasal exposure of 1-day-old newborn mice to Listeria monocytogenes (Lm). We found that nasal, but not intragastric administration, led to early CNS infection in neonate mice. In particular, upon bacterial invasion of the olfactory epithelium, Lm subsequently spread along the sensory neurons entering the brain tissue at the cribriform plate and causing a significant influx of monocytes and neutrophils. CNS infection required listeriolysin for penetration of the olfactory epithelium and ActA, a mediator of intracellular mobility, for translocation into the brain tissue. Taken together, we propose an alternative port of entry and route of infection for neonatal neurolisteriosis and present a novel infection model to mimic the clinical features of late-onset disease in human neonates.