Publikationsdatenbank

Plasmid-located dfrA14 gene in Pasteurella multocida isolates from three different pig-producing farms in Germany (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Niemann, Lisa (WE 7)

Feudi, Claudia (WE 7)

Eichhorn, Inga (WE 7)

Hanke, Dennis (WE 7)

Müller, Petra

Brauns, Jasmin

Nathaus, Rolf

Schäkel, Franziska

Höltig, Doris

Wendt, Michael

Kadlec, Kristina

Schwarz, Stefan (WE 7)

Quelle

Veterinary microbiology : an international journal

Bandzählung: 230

Seiten: 235 – 240

ISSN: 0378-1135

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S0378113518312951?via%3Dihub

DOI: 10.1016/j.vetmic.2019.01.016

Pubmed: 30827394

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Pasteurella multocida is an important respiratory tract pathogen in intensive livestock farming, especially in pigs. Antimicrobial agents are frequently used to combat infections caused by this pathogen. In a study on antimicrobial resistance among respiratory tract pathogens of pigs from 30 German pig-producing farms, P. multocida isolates (n = 9) with high minimal inhibitory concentration (MIC) values of 16/304 mg/L (n = 2), 32/608 mg/L (n = 3) or ≥64/1216 mg/L (n = 4) for trimethoprim/sulfamethoxazole (1:19) and of ≥512 mg/L (n = 9) for trimethoprim (TMP) were detected in three of these farms. The genetic relatedness of the isolates was investigated via capsule-specific PCR and macrorestriction analyses with ApaI and SmaI. Pulsed-field gel electrophoresis revealed indistinguishable restriction patterns per farm, with slight differences between the three farms. All isolates represented capsular type A. Four representative isolates, that were subjected to whole genome sequencing, shared the multi-locus sequence type (ST) 3. Their plasmids were transformed into E. coli TOP10 with subsequent selection on TMP-containing agar plates. Antimicrobial susceptibility testing and plasmid analysis of the transformants confirmed that they were resistant to sulfonamides and trimethoprim and carried only a single small plasmid. This plasmid was completely sequenced and revealed a size of 6050 bp. Sequence analyses identified the presence of a resistance gene cluster comprising the genes sul2-ΔstrA-dfrA14-ΔstrA-ΔstrB. Further analysis identified a dfrA14 gene cassette being integrated into the strA reading frame. Neither the gene dfrA14 nor this gene cluster have been detected before in P. multocida.