Publication Database

Characterization of Streptococcus pneumoniae isolates from Austrian companion animals and horses (2017)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Ginders, Maximilian

Leschnik, Michael

Künzel, Frank

Kampner, Doris

Mikula, Claudia

Steindl, Georg

Eichhorn, Inga (WE 7)

Feßler, Andrea T (WE 7)

Schwarz, Stefan (WE 7)

Spergser, Joachim

Loncaric, Igor

Quelle

Acta veterinaria Scandinavica : AVS ; official journal of the Veterinary Associations of the Nordic Countries

Bandzählung: 59

Heftzählung: 79

Seiten: 1 – 5

ISSN: 1751-0147

Sprache

Englisch

Verweise

DOI: 10.1186/s13028-017-0348-2

Pubmed: 29137652

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The aim of the present study was to investigate the genetic relatedness and the antimicrobial resistance profiles of a collection of Austrian Streptococcus pneumoniae isolates from companion animals and horses. A total of 12 non-repetitive isolates presumptively identified as S. pneumoniae were obtained during routinely diagnostic activities between March 2009 and January 2017.

Isolates were confirmed as S. pneumoniae by bile solubility and optochin susceptibility testing, matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) mass spectrometry and sequence analysis of a part recA and the 16S rRNA genes. Isolates were further characterized by pneumolysin polymerase chain reaction (PCR) and genotyped by multilocus sequence typing (MLST). Antimicrobial susceptibility testing was performed and resistance genes were detected by specific PCR assays. All isolates were serotyped. Four sequence types (ST) (ST36, ST3546, ST6934 and ST6937) and four serotypes (3, 19A, 19F and 23F) were detected. Two isolates from twelve displayed a multidrug-resistance pheno- and genotype.

This study represents the first comprehensive investigation on characteristics of S. pneumoniae isolates recovered from Austrian companion animals and horses. The obtained results indicate that common human sero- (23F) and sequence type (ST36) implicated in causing invasive pneumococcal disease (IPD) may circulate in dogs. Isolates obtained from other examined animals seem to be host-adapted.