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    Characterization of Streptococcus pneumoniae isolates from Austrian companion animals and horses (2017)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Ginders, Maximilian
    Leschnik, Michael
    Künzel, Frank
    Kampner, Doris
    Mikula, Claudia
    Steindl, Georg
    Eichhorn, Inga (WE 7)
    Feßler, Andrea T (WE 7)
    Schwarz, Stefan (WE 7)
    Spergser, Joachim
    Loncaric, Igor
    Quelle
    Acta veterinaria Scandinavica : AVS ; official journal of the Veterinary Associations of the Nordic Countries
    Bandzählung: 59
    Heftzählung: 79
    Seiten: 1 – 5
    ISSN: 1751-0147
    Sprache
    Englisch
    Verweise
    DOI: 10.1186/s13028-017-0348-2
    Pubmed: 29137652
    Kontakt
    Institut für Mikrobiologie und Tierseuchen

    Robert-von-Ostertag-Str. 7-13
    14163 Berlin
    +49 30 838 51843 / 66949
    mikrobiologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    The aim of the present study was to investigate the genetic relatedness and the antimicrobial resistance profiles of a collection of Austrian Streptococcus pneumoniae isolates from companion animals and horses. A total of 12 non-repetitive isolates presumptively identified as S. pneumoniae were obtained during routinely diagnostic activities between March 2009 and January 2017.

    Isolates were confirmed as S. pneumoniae by bile solubility and optochin susceptibility testing, matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) mass spectrometry and sequence analysis of a part recA and the 16S rRNA genes. Isolates were further characterized by pneumolysin polymerase chain reaction (PCR) and genotyped by multilocus sequence typing (MLST). Antimicrobial susceptibility testing was performed and resistance genes were detected by specific PCR assays. All isolates were serotyped. Four sequence types (ST) (ST36, ST3546, ST6934 and ST6937) and four serotypes (3, 19A, 19F and 23F) were detected. Two isolates from twelve displayed a multidrug-resistance pheno- and genotype.

    This study represents the first comprehensive investigation on characteristics of S. pneumoniae isolates recovered from Austrian companion animals and horses. The obtained results indicate that common human sero- (23F) and sequence type (ST36) implicated in causing invasive pneumococcal disease (IPD) may circulate in dogs. Isolates obtained from other examined animals seem to be host-adapted.