Publikationsdatenbank

Plasmid-located extended-spectrum β-lactamase gene bla_ROB-2 in Mannheimia haemolytica (2018)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Kadlec, Kristina

Watts, Jeffrey L.

Schwarz, Stefan (WE 7)

Sweeney, Michael T.

Quelle

The journal of antimicrobial chemotherapy : JAC

Seiten: 1 – 3

ISSN: 0305-7453

Sprache

Englisch

Verweise

DOI: 10.1093/jac/dky515

Pubmed: 30561662

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

To identify and analyse the first ESBL gene from Mannheimia haemolytica.

Susceptibility testing was performed according to CLSI. Plasmids were extracted via alkaline lysis and transferred by electrotransformation. The sequence was determined by WGS and confirmed by Sanger sequencing.

The M. haemolytica strain 48 showed high cephalosporin MICs. A single plasmid, designated pKKM48, with a size of 4323 bp, was isolated. Plasmid pKKM48 harboured a novel bla_ROB gene, tentatively designated bla_ROB-2, and was transferred to Pasteurella multocida B130 and to Escherichia coli JM107. PCR assays and susceptibility testing confirmed the presence and activity of the bla_ROB-2 gene in the P. multocida and in the E. coli recipient carrying plasmid pKKM48. The transformants had high MICs of all β-lactam antibiotics. An ESBL phenotype was seen in the E. coli transformant when applying the CLSI double-disc confirmatory test for E. coli. The bla_ROB-2 gene from plasmid pKKM48 differed in three positions from bla_ROB-1, resulting in two amino acid exchanges and one additional amino acid in the deduced β-lactamase protein. In addition to bla_ROB-2, pKKM48 harboured mob genes and showed high similarity to other plasmids from Pasteurellaceae.

This study described the first ESBL gene in Pasteurellaceae, which may limit the therapeutic options for veterinarians. The transferability to Enterobacteriaceae with the functional activity of the gene in the new host underlines the possibility of the spread of this gene across species or genus boundaries.