Publication Database

A core genome multilocus sequence typing (cgMLST) scheme for Enterococcus faecalis (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Neumann, Bernd

Prior, Karola

Bender, Jennifer K

Harmsen, Dag

Klare, Ingo

Fuchs, Stephan

Bethe, Astrid (WE 7)

Zühlke, Daniela

Göhler, André

Schwarz, Stefan (WE 7)

Schaffer, Kirsten

Riedel, Katharina

Wieler, Lothar H

Werner, Guido

Quelle

Journal of clinical microbiology

Bandzählung: 57

Heftzählung: 3

Seiten: e01686-18

ISSN: 0095-1137

Sprache

Englisch

Verweise

URL (Volltext): https://jcm.asm.org/content/jcm/57/3/e01686-18.full.pdf

DOI: 10.1128/JCM.01686-18

Pubmed: 30651394

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Among enterococci, Enterococcus faecalis occurs ubiquitously, with the highest incidence of human and animal infections. The high genetic plasticity of E. faecalis complicates both molecular investigations and phylogenetic analyses. Whole-genome sequencing (WGS) enables unraveling of epidemiological linkages and putative transmission events between humans, animals, and food. Core genome multilocus sequence typing (cgMLST) aims to combine the discriminatory power of classical multilocus sequence typing (MLST) with the extensive genetic data obtained by WGS. By sequencing a representative collection of 146 E. faecalis strains isolated from hospital outbreaks, food, animals, and colonization of healthy human individuals, we established a novel cgMLST scheme with 1972 gene targets within the Ridom SeqSphere+ software. To test the E. faecalis cgMLST scheme and assess the typing performance, different collections comprising environmental and bacteremia isolates, and all publicly available genome sequences from NCBI and SRA were analyzed. In more than 98.6% of the tested genomes, >95% good cgMLST target genes were detected (mean: 99.2% target genes). Our genotyping results not only corroborate the known epidemiological background of the isolates, but exceed previous typing resolution. In conclusion, we have created a powerful typing scheme, hence providing an international standardized nomenclature that is suitable for surveillance approaches in various sectors, linking public health, veterinary public health, and food safety in a true One Health fashion.