Publikationsdatenbank

BMP2 and TGF-ß cooperate differently during synovial-derived stem-cell Chondrogenesis in a Dexamethasone-dependent manner (2019)

Art

Poster

Autoren

Kovermann, Nikolas J. (WE 17)

Basoli, Valentina

Della Bella, Elena

Alini, Mauro

Lischer, Christoph (WE 17)

Schmal, Hagen

Kubosch, Eva Johanna

Stoddart, Martin J.

Kongress

ORS 2019 Annual Meeting Poster Sessions

Austin Convention Center, Austin Texas, 02. – 05.02.2019

Quelle

Cells : open access journal

Bandzählung: 8

Heftzählung: 6

Seiten: Article 636

ISSN: 2073-4409

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2073-4409/8/6/636

DOI: 10.3390/cells8060636

Kontakt

Pferdeklinik

Oertzenweg 19 b
14163 Berlin
+49 30 838 62299 / 62300
pferdeklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Recent studies highlighting mesenchymal stem cell (MSC) epigenetic memory suggest that a different differentiation medium may be required depending on the tissue of origin. As synovial-derived stem cells (SDSCs) attract interest we aimed to investigate the influence of TGF-β1, BMP-2 and dexamethasone on SDSC chondrogenesis in vitro. We demonstrate that dexamethasone-free medium led to enhanced chondrogenic differentiation at both the mRNA and matrix level. The greatest COL2A1/COL10A1 ratio was detected in cells exposed to a combination medium containing 10 ng/mL BMP-2 and 1 ng/mL TGF-β1 in the absence of dexamethasone, and this was reflected in the total amount of glycosaminoglycans produced. In summary, dexamethasone-free medium containing BMP-2 and TGF-β1 may be the most suitable when using SDSCs for cartilage tissue regeneration.