Publikationsdatenbank

Establishment of a murine 3D-cell-culture model of the endometrium:
A tool for investigation of early embryo implantation (2018)

Art

Poster

Autoren

Peter, Dominique (WE 11)

Michel, Geert

Na, Ellen

Wittler, Lars

Thöne-Reineke, Christa (WE 11)

Kongress

BSDB Autumn Meeting 2018, Embryonic Extrembryonic Interactions, from genetics to environment

Corpus Christi College, University of Oxford, UK, 10. – 13.09.2018

Quelle

BSDB Autumn Meeting 2018, Embryonic Extrembryonic Interactions, from genetics to environment

Sprache

Englisch

Kontakt

Institut für Tierschutz, Tierverhalten und Versuchstierkunde

Königsweg 67
14163 Berlin
+49 30 838 61146
tierschutz@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Establishment of a Murine 3D Cell Culture Model of the Endometrium
D Peter1, G Michel2, E Na3, L Wittler4, C Thöne-Reineke1

1 Institute of animal welfare, animal behavior and laboratory animal science, Freie Universität Berlin,
Berlin, Germany
2 Transgenic Technologies, Charité Universitätsmedizin Berlin, Berlin, Germany
3 Department of Cardiology, Charité Universitätsmedizin Berlin, Berlin, Germany
4 Department Developmental Genetics, Max-Planck-Institute for Molecular Genetics, Berlin, Germany

The mechanisms of embryo implantation leading to a successful pregnancy are still not entirely
understood. As human in-vivo studies are impossible, an in-vitro model is mandatory. Human primary
cells and embryonal cells are difficult to acquire. The aim of this study is to establish a murine 3D-cellculture
in-vitro model mimicking early implantation and prospectively offering the possibility to analyze
biomarkers for receptivity and embryo-endometrial-interaction. Mouse endometrial cells of CD-1 mice
are used, isolated from uteri 3.5days after mating. The isolation protocol is based on “De Clercq,
Hennes et al. 2017” and modified to necessary requirements. The endometrial epithelial cells and
stromal cells will be combined in a 3D-scaffold, physiologically layered to resemble endometrial
morphology. Addition of an embryoid body to investigate embryo-endometrial-interaction follows.
Combination of a 3D scaffold, cells and embryoid body should result in an in-vitro model for early
embryo implantation serving as a widely available investigation tool.

References: De Clercq, K., A. Hennes, lie and J. Vriens (2017). "Isolation of MouseEndometrial
Epithelial and Stromal Cells for In Vitro Decidualization." (121): e55168.