Publikationsdatenbank

Epidemiological study on tropical theileriosis (Theileria annulata infection) in the Egyptian Oases with special reference to the molecular characterization of Theileria spp (2018)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Al-Hosary, Amira

Ahmed, Laila

Ahmed, Jabbar (WE 13)

Nijhof, Ard (WE 13)

Clausen, Peter-Henning (WE 13)

Forschungsprojekt

Molecular epidemiology network for promotion and support of delivery of live vaccines against Theileria parva and Theileria annulata infection in Eastern and Northern Africa

Quelle

Ticks and tick-borne diseases

Bandzählung: 9

Heftzählung: 6

Seiten: 1489 – 1493

ISSN: 1877-959x

Sprache

Englisch

Verweise

DOI: 10.1016/j.ttbdis.2018.07.008

Pubmed: 30033328

Kontakt

Institut für Parasitologie und Tropenveterinärmedizin

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 62310
parasitologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Theileria annulata infection is a tick-borne disease known as Egyptian fever since 1947. It is a destructive obstacle for the livestock production in the Egyptian Oases (EL-Wady EL-Geded Province). The present study was conducted on 1068 cattle, ranged from below one year to more than eight years old; belonged to different farms and villages in EL-Wady EL-Geded Province. The infection was confirmed by blood smears, Tams-1 target based polymerase chain reaction (Tams-1 PCR), 18Ss rRNA polymerase chain reaction and semi nested-polymerase chain reaction (nPCR) followed by DNA sequencing and phylogenetic analyses, in addition to tick identification. Molecular techniques confirmed the infection in 63.6% (679/1068) of the examined animals while Giemsa-stained blood smears confirmed it in 36.8% (393/1062). Male and female animals showed molecular confirmed infection rates of 64.5 and 62.7%, respectively. Animals less than one year old were more infected (83.33%, 400/480) followed by animals less than three years (57.31%, 149/260) and animals less than five years (42.45%, 90/212), respectively. On the other hand, animal of five years old or above were less infected and the infection rate in this group was estimated to be 34.48% (40/116). Two tick species were identified during the present study: Hyalomma anatolicum and Rhipicephalus annulatus. Theileria annulata was the only Theileria species found in the Egyptian oases in respect to phylogenetic analysis of the obtained sequences.