Publikationsdatenbank

Analytical validation of bovine plasma ceruloplasmin measurement by p-phenylenediamine oxidation and effect of storage time and freezing temperature (2017)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Hussein, Awad Hussein

Staufenbiel, Rudolf (WE 18)

Quelle

Acta veterinaria Scandinavica : AVS ; official journal of the Veterinary Associations of the Nordic Countries

Bandzählung: 59

Heftzählung: 66

Seiten: 1 – 8

ISSN: 1751-0147

Sprache

Englisch

Verweise

DOI: 10.1186/s13028-017-0334-8

Kontakt

Nutztierklinik

Königsweg 65
14163 Berlin
+49 30 838 62261
klauentierklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background: Determination of ceruloplasmin (Cp) activity in plasma can provide an objective measure of the health
of dairy cows as well as it can be used for various diagnostic purposes. The current study was designed to perform
an analytical validation of a method for the determination of plasma Cp activity in dairy cows and to evaluate the
influences of plasma storage times and temperatures as well as freeze–thaw cycles on the activity of this enzyme. This
cohort was carried out on ten cows. For each cow, 24 aliquots of plasma, which were stored at different temperature
regimes, were prepared. Both intra- and interassay coefficients of variation (CVs) were determined. The linearity was
evaluated using bovine plasma Cp standard.
Results: The mean values of intra- and interassay CVs were 1.08 and 2.12%, respectively. Results of linearity testing
showed a high correlation coefficient (r = 0.998, P < 0.001). After 3 days of storage at room temperature and refrigeration,
the plasma activity of Cp was significantly lowered (P < 0.05). Plasma samples kept at freezing for 3 months
revealed insignificant changes in the activity of Cp. Repeated freeze–thaw cycles for three times had no significant
influence on the activity of Cp.
Conclusions: The method is easy and may be valid at values of Cp ranging from 100 to 1000 mg/L. It seems that
keeping of plasma samples at room temperature and refrigeration longer than 3 days is unsuitable for Cp measurement.
In addition, Cp remains stable in plasma samples stored at freezing as well as repeat freeze–thaw cycles.