Publication Database

Detecting Staphylococcus aureus in milk from dairy cows using sniffer dogs (2018)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Fischer-Tenhagen, C (WE 19)

Theby, V

Krömker, V

Heuwieser, W (WE 19)

Quelle

Journal of dairy science : JDS

Bandzählung: 101

Heftzählung: 5

Seiten: 4317 – 4324

ISSN: 0022-0302

Sprache

Englisch

Verweise

DOI: 10.3168/jds.2017-14100

Pubmed: 29501329

Kontakt

Tierklinik für Fortpflanzung

Königsweg 65
Haus 27
14163 Berlin
+49 30 838 62618
fortpflanzungsklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Fast and accurate identification of disease-causing pathogens is essential for specific antimicrobial therapy in human and veterinary medicine. In these experiments, dogs were trained to identify Staphylococcus aureus and differentiate it from other common mastitis-causing pathogens by smell. Headspaces from agar plates, inoculated raw milk samples, or field samples collected from cows with Staphylococcus aureus and other mastitis-causing pathogens were used for training and testing. The ability to learn the specific odor of Staphylococcus aureus in milk depended on the concentration of the pathogens in the training samples. Sensitivity and specificity for identifying Staphylococcus aureus were 91.3 and 97.9%, respectively, for pathogens grown on agar plates; 83.8 and 98.0% for pathogens inoculated in raw milk; and 59.0 and 93.2% for milk samples from mastitic cows. The results of these experiments underline the potential of odor detection as a diagnostic tool for pathogen diagnosis.