Publikationsdatenbank

Exploration of serum- and cell culture-derived exosomes from dogs (2018)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Aguilera-Rojas, Matias (WE 3)

Badewien-Rentzsch, Brit

Plendl, Johanna (WE 1)

Kohn, Barbara (WE 20)

Einspanier, Ralf (WE 3)

Quelle

BMC veterinary research

Bandzählung: 14

Heftzählung: 1

Seiten: 179

ISSN: 1746-6148

Sprache

Englisch

Verweise

URL (Volltext): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994050/pdf/12917_2018_Article_1509.pdf

DOI: 10.1186/s12917-018-1509-x

Pubmed: 29884196

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Exosomes are defined as extracellular membrane vesicles, 30-150 nm in diameter, derived from all types of cells. They originate via endocytosis and then they are released through exocytosis to the extracellular space, being found in various biological fluids as well as in cell culture medium. In the last few years, exosomes have gained considerable scientific interest due to their potential use as biomarkers, especially in the field of cancer research. This report describes a method to isolate, quantify and identify serum- and cell culture-derived exosomes from dog samples, using small volumes (100 μL and 1 mL, respectively).

Quantification and sizing of exosomes contained in serum and cell culture samples were assessed by utilizing nanoparticle tracking analysis, transmission electron microscopy and immunoelectron microscopy. Detected particles showed the normal size (30-150 nm) and morphology described for exosomes, as well as presence of the transmembrane protein CD63 known as exosomal marker.

Based on a validated rapid isolation procedure of nanoparticles from small volumes of different types of dog samples, a characterization and exploration of intact exosomes, as well as facilitation for their analysis in downstream applications was introduced.